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Raji cell lysate was probed with anti-Bcl-2 (1μg/ml).
Anti-Bcl2, clone 100
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, Ca, Fe | FC, IH, WB | Mouse | Purified | Monoclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: P10415 # Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).
SIZE: 239 amino acids; 26266 Da
SUBUNIT: Forms h ... see more »
SIZE: 239 amino acids; 26266 Da
SUBUNIT: Forms h ... see more »
Entrez Gene Number:
Entrez Gene Summary
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.
Description:
Anti-Bcl2, clone 100
Background Information:
The Bcl name is derived from B-cell chronic lymphocytic leukemia, and was given to genes involved in that disease. Members of the Bcl2 family share homologous domains (BH) through which they are able to homo- and hetero-dimerize. Apoptosis/survival is regulated by the relative balance of pro-apoptotic (Bax, BclXS, Bak, Bad) and anti-apoptotic (Bcl2, BclXL, Mcl-1, Dad-1) family members, and their heterodimerization. Bcl2 plays an important role in cytochrome c release from the mitochondria; it also binds Apaf1, inhibiting Apaf-1 association with caspase-9. Bcl-2 overexpression in indicated in a number of human cancers, including melanoma, breast, prostate, and lung, and it correlates with resistance to cancer treatment (presumably due to decreased apoptosis). Bcl-2 has also been implicated in schizophrenia and autoimmune disorders.
Alternate Names:
- B-cell CLL/lymphoma 2
- B-cell lymphoma protein 2
Clone:
100
Specificity:
This antibody recognizes Bcl2, Mr 26 kDa.
Key Applications:
- Flow Cytometry
- Immunohistochemistry
- Western Blotting
Application Notes:
Immunohistochemistry:
An independent laboratory has reported that this antibody detects Bcl2 in paraffin and acetone-fixed tissues.
FACS Analysis:
Recommended
An independent laboratory has reported that this antibody detects Bcl2 in paraffin and acetone-fixed tissues.
FACS Analysis:
Recommended
Species Reactivity:
- Human
- Canine
- Feline
Species Reactivity Note:
Human. Predicted to cross-react with feline and canine, based on sequence homology.
Isotype:
IgG1
Immunogen:
Synthetic peptide corresponding to amino acids 41-54 of human Bcl2 protein. The immunizing sequence is identical in canine and feline.
Epitope:
a.a. 41-54
Molecular Weight:
26 kDa
Control:
Tonsil tissue.
Presentation:
Purified mouse monoclonal IgG1 in buffer containing 70% storage buffer PBS with 0.1% sodium azide and 30% glycerol. Store at -20ºC.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20ºC may cause glycerol-containing solutions to become frozen during storage.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20ºC may cause glycerol-containing solutions to become frozen during storage.
Gene Symbol:
- BCL2
- Bcl-2
Quality Assurance:
Routinely evaluated by western blot on Raji RIPA cell lysate.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected Bcl2 in a Raji RIPA cell lysate.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected Bcl2 in a Raji RIPA cell lysate.
Antibody Category:
Apoptosis & Cancer
Antibody Sub-Category:
BCL2 & Inhibition
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Upstate (Millipore)
Format Code:
Pur
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody
Format:
Purified
Host:
Mouse
Purification Method:
Protein G purified