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WB: 20 micrograms of untreated (Lanes 1 and 3) or PDGF stimulated (lanes 2 and 4) NIH-3T3 cell lysate was probed with anti-phospho-Akt1/PKBα (Ser473)...
Anti-phospho-Akt1/PKBα (Ser473), clone SK703
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB | Rabbit | Purified | Monoclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: P31749 # General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surfac
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Entrez Gene Number:
Entrez Gene Summary
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuro
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Description:
Anti-phospho-Akt1/PKBα (Ser473), clone SK703
Specificity:
phosphorylated Akt1/PKBa
Key Applications:
Western Blotting
Species Reactivity:
- Human
- Mouse
Isotype:
IgG
Immunogen:
Peptide containing a pSer that corresponds to amino acid position 473 of human Akt1/PKBa
Modifications:
Phosphorylation
Molecular Weight:
60kDa
Presentation:
of 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
Storage Conditions:
2 years at -20°C
Gene Symbol:
- AKT1
- RAC
- PRKBA
- RAC-ALPHA
- PKB
- MGC99656
- RAC-PK-alpha
- C-AKT
- AKT
Quality Assurance:
routinely evaluated by immunoblot on lysates from mouse NIH-3T3 fibroblasts treated with PDGF
Antibody Category:
Signaling
Antibody Sub-Category:
PI3K, Akt, & mTOR Signaling
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Upstate (Millipore)
Format:
Purified
Host:
Rabbit
Antibody Type:
Monoclonal Antibody
Purification Method:
protein A purified