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WB: 20 micrograms of crude cell extracts (lanes 1 & 2) or ATM-containing immune complexes (lanes 3 & 4) from either untreated (lanes 1 & 3) or γ-irra...
Anti-phospho-ATM (Ser1981), clone 10H11.E12
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | IC, IF, IP, WB | Mouse | Purified | Monoclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: Q13315 # Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also involved in signal transduction and cell cycle control. M
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Entrez Gene Number:
Entrez Gene Summary
The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that
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Description:
Anti-phospho-ATM (Ser1981), clone 10H11.E12
Background Information:
Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are related kinases that regulate cell cycle checkpoints and DNA repair. Mutation in the ATM gene results in the autosomal recessive disease ataxia telangiectasia (AT). The identified substrates for ATM are p53, p95/NBS1, MDM2, Chk2, BRCA1, CtIP, 4E-BP1 and Chk1. The essential requirement for the substrates of ATM/ATR is S/TQ. Hydrophobic amino acids at positions -3 and -1, and negatively charged amino acids at position +1 are positive determinants for substrate recognition by these kinases. Positively charged residues surrounding the S/TQ are negative determinants for substrate phosphorylation. The complex phenotype of cells derived from patients with AT suggests that ATM has additional cellular substrates. In unirradiated cells, ATM is present as an inactive homodimer or multimer. Double-stranded breaks in DNA caused by ionizing radiation cause rapid ATM kinase activation through dissociation of this complex and ATM autophosphorylation at Ser1981.
Alternate Names:
- A-T, mutated
- AT mutated
- TEL1, telomere maintenance 1, homolog
- ataxia telangiectasia mutated
- ataxia telangiectasia mutated (includes complementation groups A, C and D)
- ataxia telangiectasia mutated protein
- human phosphatidylinositol 3-kinase homolog
- serine-protein kinase ATM
Clone:
10H11.E12
Specificity:
This antibody recognizes ATM, Mr ~370 kDa. A non-specific protein was also detected, Mr ~ >400 kDa.
Key Applications:
- Immunocytochemistry
- Immunofluorescence
- Immunoprecipitation
- Western Blotting
Application Notes:
Immunoprecipitation:
Phosphorylated ATM was immunoprecipitated from irradiated HeLa cells (Figure A, lanes 3 and 4).
Immunocytochemistry:
Foci are detected in irradiated human and mouse fibroblasts. Determined by an independent laboratory.
Phosphorylated ATM was immunoprecipitated from irradiated HeLa cells (Figure A, lanes 3 and 4).
Immunocytochemistry:
Foci are detected in irradiated human and mouse fibroblasts. Determined by an independent laboratory.
Species Reactivity:
- Human
- Mouse
Species Reactivity Note:
Human and mouse. Predicted to cross-react with rat based on sequence homology
Isotype:
IgG1κ
Immunogen:
KLH-conjugated, synthetic peptide corresponding to amino acids 1974-1988 (SLAFEEG[pS]QSTTISS) of human ATM. The immunizing sequence has 11/12 identical amino acids in mouse and rat.
Modifications:
Phosphorylation
Molecular Weight:
~370 kDa
Control:
Irradiated HeLa cell lysates
Presentation:
Protein G purified mouse IgG in 0.014 M phosphate buffer, pH 7.6, with 0.175 M NaCl, 0.07 % Sodium Azide and 30% glycerol. Liquid at -20°C.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Gene Symbol:
- ATM
- AT
- TELO1
- ATC
- DKFZp781A0353
- MGC74674
- ATD
- ATA
- AT1
- T-PLL
- ATE
- TEL1
- ATDC
- TPLL
Quality Assurance:
Routinely evaluated by immunoblot on in crude lysates from irradiated HeLa cells.
Western Blot Analysis:
0.5 µg/mL of this lot detected phosphorylated ATM in crude lysates from irradiated HeLa cells.
Western Blot Analysis:
0.5 µg/mL of this lot detected phosphorylated ATM in crude lysates from irradiated HeLa cells.
Antibody Category:
Epigenetics & Nuclear Function
Antibody Sub-Category:
Cell Cycle, DNA Replication & Repair
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Upstate (Millipore)
Format Code:
Pur
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody
Format:
Purified
Host:
Mouse
Purification Method:
Protein G purified