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  • : 05-747
  • : 100 µL

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WB: 20 micrograms of untreated (Lanes 1 & 3) and B-Raf phosphorylated (Lanes 2 & 4) PC-12 cell lysate was probed with a 1:5000 dilution of Anti-phosph...

Anti-phospho-MEK1 (Ser218/222)/MEK2 (Ser222/226) Antibody, rabbit monoclonal

Species Reactivity Key Applications Host Format Antibody Type
H, M, R WB Rabbit Purified Monoclonal Antibody
Description:
Anti-phospho-MEK1 (Ser218/222)/MEK2 (Ser222/226) Antibody, rabbit monoclonal
Promotional Text:
Trade Name:
Upstate (Millipore)
Specificity:
phosphorylated MEK1/MEK2
Molecular Weight:
45kDa
Immunogen:
Peptide containing DpSMANpSF in which pS corresponds to phosphorylated amino acids 218 and 222 of human MEK1
Modifications:
Phosphorylation
Isotype:
Species Reactivity:
  • Human
  • Mouse
  • Rat
Quality Assurance:
routinely evaluated by immunoblot on RIPA lysates from PC-12 cells that have been treated with B-Raf
Purification Method:
Protein A purfied
Presentation:
0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
Storage Conditions:
2 years at -20°C
UniProt Number:
Entrez Gene Number:
Gene Symbol:
  • MAP2K1
  • PRKMK1
  • MKK1
  • MAPKK1
  • MEK1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Western Blotting
Entrez Gene Summary:
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.
UniProt Summary:
FUNCTION: SwissProt: Q02750 # Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
SIZE: 393 amino acids; 43439 Da
SUBUNIT: Interacts with MORG1 (By similarity). Interacts with Yersinia yopJ.
PTM: Phosphorylation on Ser/Thr by MAP kinase kinase kinases (RAF or MEKK1) regulates positively the kinase activity. & Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
DISEASE: SwissProt: Q02750 # Defects in MAP2K1 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio- cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
SIMILARITY: SwissProt: Q02750 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. & Contains 1 protein kinase domain.
Brand Family:
Upstate
Product Name:
Anti-phospho-MEK1 (Ser218/222)/MEK2 (Ser222/226) Antibody, rabbit monoclonal
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 µL
Format:
Purified
Host:
Rabbit
Applications:
Detect phospho-MEK1 (Ser218/222)/MEK2 (Ser222/226) using this Anti-phospho-MEK1 (Ser218/222)/MEK2 (Ser222/226) Antibody validated for use in WB.

Product Resources

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