Ordering Information
- : 05-777
- : 1 mg
Product Images
Anti-Phosphotyrosine Antibody, recombinant clone 4G10® | 05-777
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Vrt | IP, WB | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-Phosphotyrosine Antibody, recombinant clone 4G10® | 05-777
Promotional Text:
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes tyrosine-phosphorylated proteins from all species.
Molecular Weight:
varies
Immunogen:
Produced from CHO cells expressing the 4G10 antibody heavy and light chain cDNAs. Heavy chain C-terminus has a hexa-histidine tag for purification and immobilization via Nickel affinity matrices.
Modifications:
Phosphorylation
Clone:
4G10®
Isotype:
IgG2bκ
Background Information:
Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine.). Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. The advent of anti-phospho-tyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.
Species Reactivity:
Vertebrates
Control:
Positive Control Included: EGF stim A431 Cell Lysate (12-302).
Quality Assurance:
routinely evaluated by immunoblot on a modified RIPA lysate from EGF-treated human A431 carcinoma cells
Purification Method:
Protein G purfied
Presentation:
Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
Storage Conditions:
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Immunoprecipitation
- Western Blotting
Brand Family:
Upstate
Product Name:
Anti-Phosphotyrosine Antibody, recombinant clone 4G10® | 05-777
Antibody Type:
Monoclonal Antibody
Qty/Pk:
1 mg
Format:
Purified
Host:
Mouse