Ordering Information
- : 05-784R
- : 100 μL
- : 0.1 mg/mL
Product Images
Immunoprecipitation Analysis:
Representative lot data.
1 µg from a previous lot immunoprecipitated IRS-1 from 100 µg of MCF7 cell lysate. ...
Western Blot (Snap ID) Analysis:
Representative lot data.
3T3/A31 (lane 1),3T3/L1 (lane 2), L6 (lane 3), and MCF7 (lane 4) lysates were r...
. There is cross reactivity of the detaction antibody with Rb IgG HC as shown at ~50 kDa.'))
 Analysis: <br />Representative lot data. <br />3T3/A31 (lane 1),3T3/L1 (lane 2), L6 (lane 3), and MCF7 (lane 4) lysates were resolved by electrophoresis, transferred to PVDF membranes and probed with Anti-IRS1, clone 58-10C-31 (1:1000) dilution. <br />Proteins were visualized using a Donkey Anti-Rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. <br />Arrow indicates IRS1 (~160 kDa).'))
, 3T3/L1 (lane 2), L6 (lane 3), and MCF7 (lane 4) lysates were resolved by electrophoresis, transferred to PVDF membranes and probed with Anti-IRS1, clone 58-10C-31 (1:1000) dilution. <br />Proteins were visualized using a Donkey Anti-Rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. <br />Arrow indicates IRS1 (~160 kDa).'))
Anti-IRS1, clone 58-10C-31
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R, H | WB, IP | Rabbit | Purified | Monoclonal Antibody |
Description:
Anti-IRS1 Antibody, clone 58-10C-31 | 05-784R
Promotional Text:
Specificity:
Antibody recognizes the C-terminus of IRS1.
Molecular Weight:
~ 160 kDa
Epitope:
C-terminus
Immunogen:
KLH- conjugated - linear peptide corresponding to the C-terminus of rat IRS1.
Clone:
58-10C-31
Isotype:
IgG
Background Information:
IRS1 (Insulin Receptor Substrate 1) transmits insulin signals via metabolic and mitogenic pathways. It is heavily phosphorylated on both serine and tyrosine residues. These phosphorylated tyrosines enable IRS to act as a docking protein that binds SH2 domains of such proteins as PI3 Kinase (phosphatidylinositol 3-kinase) and GRB2, resulting in activation. Over expression and phosphorylation of serine is associated with insulin resistance and breast cancer.
Species Reactivity:
- Mouse
- Rat
- Human
Species Reactivity Note:
Reacts with mouse, rat and human.
Application Notes:
Western Blot (SNAP ID) Analysis: 0.1 µg/mL from a previous lot detected IRS-1 on 10 µg of 3T3/A31, 3T3/L1, L6 and MCF7 cell lysates.
Immunoprecipitation Analysis: 1 µg from a previous lot immunoprecipitated IRS-1 from 100 µg of MCF7 cell lysate. Arrow indicates IRS-1 (~160 kDa). There is cross reactivity of the detection antibody with the heavy chaing of Rabbit IgG as shown at ~50 kDa.
Immunoprecipitation Analysis: 1 µg from a previous lot immunoprecipitated IRS-1 from 100 µg of MCF7 cell lysate. Arrow indicates IRS-1 (~160 kDa). There is cross reactivity of the detection antibody with the heavy chaing of Rabbit IgG as shown at ~50 kDa.
Control:
3T3/A31, 3T3/L1, L6, or MCF7 cell lysates
Quality Assurance:
Evaluated by Western Blot in 3T3/A31, 3T3/L1, L6 or MCF7 cell lysates.
Western Blot Analysis: 0.1 µg/mL of this antibody detected IRS-1 on 10 µg of 3T3/A31, 3T3/L1, L6, or MCF7 cell lysates.
Western Blot Analysis: 0.1 µg/mL of this antibody detected IRS-1 on 10 µg of 3T3/A31, 3T3/L1, L6, or MCF7 cell lysates.
Purification Method:
Protein A purfied
Presentation:
Purified Rabbit Monoclonal IgG Supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide and 40% glycerol.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- IRS1
- IRS-1
- HIRS-1
Alternate Names:
insulin receptor substrate 1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Western Blotting
- Immunoprecipitation
UniProt Summary:
FUNCTION: May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit. Ref.3
SUBUNIT STRUCTURE: Interacts with SOCS7 By similarity. Interacts with the NPXY motif of tyrosine-phosphorylated IGF1R and INSR via the PTB domain. Binds to phosphatidylinositol 3-kinase p85 subunit via the phosphorylated YXXM motifs. Binds ROCK1.
PTM: Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-307 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor, and Ser-789 phosphorylation is increased in the liver of insulin-resistant rats.
Phosphorylation of Tyr-895 is required for GRB2-binding.
SEQUENCE SIMILARITIES: Contains 1 IRS-type PTB domain.
Contains 1 PH domain.
SUBUNIT STRUCTURE: Interacts with SOCS7 By similarity. Interacts with the NPXY motif of tyrosine-phosphorylated IGF1R and INSR via the PTB domain. Binds to phosphatidylinositol 3-kinase p85 subunit via the phosphorylated YXXM motifs. Binds ROCK1.
PTM: Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-307 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor, and Ser-789 phosphorylation is increased in the liver of insulin-resistant rats.
Phosphorylation of Tyr-895 is required for GRB2-binding.
SEQUENCE SIMILARITIES: Contains 1 IRS-type PTB domain.
Contains 1 PH domain.
Product Name:
Anti-IRS1, clone 58-10C-31
Concentration:
0.1 mg/mL
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 μL
Format:
Purified
Host:
Rabbit