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画像情報
IB: Jurkat cell lysate was probed with anti-CD3ε(0.5μg/ml).
T-cell Activation by an Antigen presenting Cell. Panel A is an image of a single Antigen-Presenting Cell (APC; a Raji cell, stained blue...
Anti-CD3ε, clone APA1/1
当該製品は右記製品の推奨代替品です: CBL1302| 交差性 | 主要アプリケーション | 免疫動物 | フォーマット | 抗体タイプ |
|---|---|---|---|---|
| H, M | FC, IP, WB, IC | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-CD3ε, clone APA1/1
Replaces:
CBL1302
Trade Name:
Upstate (Millipore)
Specificity:
CDε
Molecular Weight:
~23kDa
Immunogen:
Purified human CD3 epsilon
Isotype:
IgG1
Species Reactivity:
- Human
- Mouse
Application Notes:
Clone APA1/1 has been demonstrated to recognize a site on CD3&epsilon: that is exposed upon engagement of the T-Cell Receptor complex. Exposure of this neoepitope precedes CD3 phosphorylation and recruitment and activation of ZAP-70, which initiates the signaling cascade produced by T-cell activation. As such, clone APA1/1 provides the earliest known marker for T-Cell Receptor engagement, and thus T-cell activation.
Quality Assurance:
routinely evaluated by immunoblot on RIPA lysates from Jurkat cells
Purification Method:
Protein G purfied
Presentation:
0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide
Storage Conditions:
1 year at 2-8°C from date of shipment.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- CD3E
- T3E
- TCRE
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Flow Cytometry
- Immunoprecipitation
- Western Blotting
- Immunocytochemistry
Entrez Gene Summary:
The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women.
UniProt Summary:
FUNCTION: SwissProt: P07766 # The CD3 complex mediates signal transduction.
SIZE: 207 amino acids; 23147 Da
SUBUNIT: The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
SIMILARITY: SwissProt: P07766 ## Contains 1 Ig-like (immunoglobulin-like) domain. & Contains 1 ITAM domain.
SIZE: 207 amino acids; 23147 Da
SUBUNIT: The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
SIMILARITY: SwissProt: P07766 ## Contains 1 Ig-like (immunoglobulin-like) domain. & Contains 1 ITAM domain.
Brand Family:
Upstate
Product Name:
Anti-CD3ε, clone APA1/1
Antibody Type:
Monoclonal Antibody
Qty/Pk:
200 µg
Format:
Purified
Host:
Mouse