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IB: Jurkat cell lysate was probed with anti-CD3ε(0.5μg/ml).
T-cell Activation by an Antigen presenting Cell. Panel A is an image of a single Antigen-Presenting Cell (APC; a Raji cell, stained blue...
Anti-CD3ε, clone APA1/1
Recommended Replacement for: CBL1302| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | FC, IP, WB, IC | Mouse | Purified | Monoclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: P07766 # The CD3 complex mediates signal transduction.
SIZE: 207 amino acids; 23147 Da
SUBUNIT: The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
SIMILARITY: SwissProt: P07766 ## Contains 1 I ... see more »
SIZE: 207 amino acids; 23147 Da
SUBUNIT: The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
SIMILARITY: SwissProt: P07766 ## Contains 1 I ... see more »
Entrez Gene Number:
Entrez Gene Summary
The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role
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Description:
Anti-CD3ε, clone APA1/1
Replaces:
CBL1302
Specificity:
CDε
Key Applications:
- Flow Cytometry
- Immunoprecipitation
- Western Blotting
- Immunocytochemistry
Application Notes:
Clone APA1/1 has been demonstrated to recognize a site on CD3&epsilon: that is exposed upon engagement of the T-Cell Receptor complex. Exposure of this neoepitope precedes CD3 phosphorylation and recruitment and activation of ZAP-70, which initiates the signaling cascade produced by T-cell activation. As such, clone APA1/1 provides the earliest known marker for T-Cell Receptor engagement, and thus T-cell activation.
Species Reactivity:
- Human
- Mouse
Isotype:
IgG1
Immunogen:
Purified human CD3 epsilon
Molecular Weight:
~23kDa
Presentation:
0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
Storage Conditions:
2 years at -20°C
Gene Symbol:
- CD3E
- T3E
- TCRE
Quality Assurance:
routinely evaluated by immunoblot on RIPA lysates from Jurkat cells
Antibody Category:
Inflammation & Immunology
Antibody Sub-Category:
Immunoglobulins & Immunology
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Upstate (Millipore)
Antibody Type:
Monoclonal Antibody
Format:
Purified
Host:
Mouse
Purification Method:
protein G purified