Anti-phospho-Erk1/2 (Thr202/Tyr204, Thr185/Tyr187), recombinant clone AW39R

P28482

FUNCTION: SwissProt: P28482 # Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
COFACTOR: Magnesium (By similarity).
SIZE: 360 amino acids; 41390 Da
SUBUNIT: Interacts with MORG1 (By similarity). Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine- kinase activity. Interacts with HSF4.
DOMAIN: SwissProt: P28482 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
PTM: Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme.
SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain. ... hide » see more »

NP_002736

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. ... hide » see more »

Anti-phospho-Erk1/2 (Thr202/Tyr204, Thr185/Tyr187), recombinant clone AW39R

05-797

Erk (Extracellular signal-Related Kinase) is a family of two, highly homologous proteins denoted as Erk1 (p44, MAPK3) and Erk2 (p42, MAPK1) that both function in the same pathway. The two proteins are often referred to collectively as Erk1/2 or p44/p42 MAP kinase. The Erk pathway is considered the classical, canonical MAPK (Mitogen-Activated Protein Kinase) signaling pathway. It is an evolutionarily conserved pathway that controls and is a critical regulator of the growth and survival through the promotion of cell proliferation and the prevention of apoptosis. Erk is involved in the control of many fundamental cellular processes including cell proliferation, survival, differentiation, apoptosis, motility and metabolism. Erk is activated by growth factor stimulation of receptor tyrosine kinases (RTKs), GPCR, and/or integrin stimulation. This activates the Ras-Raf-MEK-Erk pathway that results in the phosphorylation/activation of Erk1/2 (p44/p42) on the TxY motif (Thr202/Tyr204 and Thr185/Tyr187 for Erk1 & Erk2, respectively).

• Erk1
• Erk2
• p44-MAPK
• p42-MAPK
• MAPK2
• PRKM2
• p41mapk
• PRKM1
• p44
• ERK2
• P42MAPK
• ERK
• p42
• MAPK1
• ERT1
• ERT2
• Mitogen-activated protein kinase
• MAP Kinase 2
• MAP Kianse 1
• MAPK

AW39R

Recognizes Erk 1 & 2 only when dually phosphorylated on its TxY activation motif.

• Multiplexing
• Western Blotting

• Human
• Mouse
• Rat

IgG

Phosphorylated peptide corresponding to amino acids surrounding the pTEpY motif in the activation loop of human phospho-Erk1/2, in which the Thr and Tyr residues are phosphorylated

pTEpY in the activation loop

Phosphorylation

Erk1 (p44) (~44 kDa) and Erk2 (p42) (~42 kDa).

PDGF-treated NIH/3T3 cell lysates

Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

• Erk1
• Erk2
• p44-MAPK
• p42-MAPK
• MAPK2
• PRKM2
• p41mapk
• PRKM1
• p44
• ERK2
• P42MAPK
• ERK
• p42
• MAPK1
• ERT1
• ERT2
• Mitogen-activated protein kinase
• MAP Kinase 2
• MAP Kianse 1
• MAPK

Routinely evaluated by immunoblot on lysate from PDGF-treated NIH/3T3 cells

Signaling

• MAP Kinases
• Kinases & Phosphatases

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Upstate (Millipore)

Pur

• MAPK
• Cancer

Monoclonal Antibody

Purified

Rabbit

Protein A Purified