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Ordering Information

  • : 05-831-I
  • : 100 µL
  • : Please refer to the Certificate of Analysis for the lot-specific concentration.

Product Images

ELISA Analysis:
Representative lot data.
The above graph displays an antibody titer using Cat. No. 05-831-I, Anti-Amyloid Beta (ABeta) x-42....

Immunohistochemistry Analysis:
Representative lot data.
Formalin Fixed Paraffin Embedded (FFPE) human Alzheimer's disease brain tissue was p...

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Anti-Amyloid Beta (ABeta) x-42, clone 12F4

Recommended Replacement for: 05-831
Species Reactivity Key Applications Host Format Antibody Type
H IHC, ELISA Mouse Purified Monoclonal Antibody
Description:
Anti-Amyloid Beta (ABeta) x-42 Antibody, clone 12F4
Replaces:
05-831
Promotional Text:
Specificity:
This antibody detects Amyloid Beta (ABeta) x-42, but does not cross react with Amyloid Beta (ABeta) x-40 or Amyloid Beta (Abeta) x-43.
Molecular Weight:
4 kDa calculated
Immunogen:
KLH-conjugated linear peptide corresponding to human Amyloid Beta (ABeta) x-42.
Clone:
Isotype:
IgG1κ
Background Information:
One of the most important and initial steps which causes loss of memory and cognition in Alzheimer’s Disease (AD) involves proteolytic cleavage of amyloid precursor protein (APP, chromosome 21) releasing short 40, 42 & 43 amino acid peptides (Amyloid Beta) 1-40, 1-42 and 1-43). Polymerization of beta-amyloid and subsequent neuronal deposit (amyloid) leads to the degeneration of neurons involved in memory and cognition.
Species Reactivity:
Human
Application Notes:
Immunohistohemistry Analysis: A 1:500 dilution from a representative lot detected Amyloid Beta (ABeta) x-42 in human pons/midbrain tissue.

ELISA Analysis: A representative lot detected Amyloid Beta (ABeta) x-42, but demonstrates a loss of signal against Amyloid Beta (ABeta) x-42.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Amyloid Beta (ABeta) x-42, but demonstrated a loss of signal against x-40 and not x-43 in WB (Prof. J. Buxbaum, Mt. Sinai School of Medicine).
Control:
Human Alzheimer's brain tissue.
Quality Assurance:
Evaluated by Immunohistochemistry in human Alzheimer's disease brain tissue.

Immunohistochemistry Analysis: A 1:2,000 dilution of this antibody detected Amyloid Beta (Abeta) x-42 in human Alzheimer's disease brain tissue.
Purification Method:
Protein G purified
Presentation:
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
  • APP
  • A4
  • AD1
Alternate Names:
  • Amyloid beta A4 protein
  • ABPP
  • APPI
  • APP
  • Alzheimer disease amyloid protein
  • Cerebral vascular amyloid peptide
  • CVAP
  • PreA4
  • Protease nexin-II
  • PN-II
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
  • Immunohistochemistry
  • Enzyme Immunoassay (ELISA)
Product Name:
Anti-Amyloid Beta (ABeta) x-42, clone 12F4
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 µL
Format:
Purified
Host:
Mouse
Applications:
Anti-Amyloid Beta (ABeta) x-42, clone 12F4 is an antibody targeting the Amyloid Beta (ABeta) x-42 protein, validated for use in IHC & ELISA.

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