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Phoenix cells (293T plus) were transfected with an expression vector encoding IDO. The transfected cells were mixed with a large number of untransfect...
WB: Unstimulated (lane 1) and human IFN-gamma stimulated (lane 2) HeLa cell lysates were probed with anti-IDO (Indoleamine 2,3-Dioxygenase) (0.5μg/ml)
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Anti-IDO (Indoleamine 2,3-Dioxygenase), clone 10.1
Recommended Replacement for: 05-840 is a recommended replacement for AB5968| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | IC, WB | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-IDO (Indoleamine 2,3-Dioxygenase), clone 10.1
Replaces:
05-840 is a recommended replacement for AB5968
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes IDO (Indoleamine 2,3- Dioxygenase), Mr 42kDa.
Molecular Weight:
42 kDa
Immunogen:
GST fusion protein corresponding to residues 78-184 of human Indoleamine 2,3- Dioxygenase (IDO). Clone 10.1.
Clone:
10.1
Isotype:
IgG
Background Information:
Indoleamine 2,3-dioxygenase (IDO) is an enzyme that is responsible for converting tryptophan to kynurenines. IDO is expressed by a wide variety of tissues and IDO can be upregulated by interferon gamma. IDO modulates levels of the amino acid tryptophan, which is vital for cell growth, but is also involved in the suppression of the immune response. IDO may be involved in the suppression of the immune response to tumours and blocking the IDO pathway may be a potential target for immunotherapy.
Species Reactivity:
- Human
- Mouse
Species Reactivity Note:
Human and mouse.
Application Notes:
Immunocytochemistry
Control:
Human IFN-gamma stimulated HeLa cell lysates, IFN gamma stimulated human peripheral blood lymphocytes.
Quality Assurance:
Routinely evaluated by Western Blot on untreated and IFNgamma treated (24 hrs) Hela lysates.
Western Blot Analysis:
1:500 dilution of this lot detected IDO on 10 ug of untreated and IFN gamma treated Hela lysates.
Western Blot Analysis:
1:500 dilution of this lot detected IDO on 10 ug of untreated and IFN gamma treated Hela lysates.
Purification Method:
Protein G purfied
Presentation:
Purified mouse monoclonal IgG in buffer containing 70% storage buffer 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide and 30% glycerol.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- INDO
- IDO
Alternate Names:
- Indoleamine 2,3-dioxygenase
- Indoleamine-pyrrole 2,3-dioxygenase
- indole 2,3-dioxygenase
- indoleamine-pyrrole 2,3 dioxygenase
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Immunocytochemistry
- Western Blotting
Entrez Gene Summary:
Gamma-interferon (IFNG; MIM 147570) has an antiproliferative effect on many tumor cells and inhibits intracellular pathogens such as Toxoplasma and Chlamydia, at least partly because of the induction of indoleamine 2,3-dioxygenase (INDO; EC 1.13.11.42). This enzyme catalyzes the degradation of the essential amino acid L-tryptophan to N-formylkynurenine.[supplied by OMIM]
UniProt Summary:
FUNCTION: SwissProt: P14902 # Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen.
COFACTOR: Binds 1 heme group per subunit.
SIZE: 403 amino acids; 45326 Da
SIMILARITY: SwissProt: P14902 ## Belongs to the indoleamine 2,3-dioxygenase family.
COFACTOR: Binds 1 heme group per subunit.
SIZE: 403 amino acids; 45326 Da
SIMILARITY: SwissProt: P14902 ## Belongs to the indoleamine 2,3-dioxygenase family.
Brand Family:
Upstate
Product Name:
Anti-IDO (Indoleamine 2,3-Dioxygenase), clone 10.1
Concentration:
0.54 mg/mL
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 µg
Format:
Purified
Host:
Mouse