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Ordering Information
- : 05-850
- : 200 µg
Product Images
WB: 20 micrograms of HeLa nuclear extract was probed with Anti-p68, clone PAb204 (0.2μg/ml).
Anti-p68, clone PAb204
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-p68, clone PAb204
Trade Name:
Upstate (Millipore)
Product Overview:
Also known as DDX5, p68 is a DEAD-box ATPase and RNA splicing factor.
Specificity:
p68
Molecular Weight:
68kDa
Immunogen:
Purified T antigen-related protein from Ad2+ ND2 virus-infected HeLa cells.
Isotype:
IgG1
Species Reactivity:
- Human
- Mouse
- Rat
Quality Assurance:
Routinely evaluated by immunoblot of nuclear extracts from HeLa cells
Purification Method:
Protein G purfied
Storage Conditions:
2 years at -20°C
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- DDX5
- HUMP68
- DKFZp686J01190
- p68
- HLR1
- HELR
- G17P1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified.
Key Applications:
Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P17844 # RNA-dependent ATPase activity. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. May be involved in pre-mRNA splicing.
SIZE: 614 amino acids; 69148 Da
SUBUNIT: Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRPA1, HNRPA2B1, HNRPA3, HNRPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRPR, HNRPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SFRS1, SKIV2L2, SNRPA1, SNRPB, SNRPB2, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, SNW1, SRRM1, SRRM2, SYF2, SYNCRIP, TFIP11, THOC4, U2AF1, WDR57, XAB2 and ZCCHC8.
SUBCELLULAR LOCATION: Nucleus, nucleolus.
PTM: Arg-502 is dimethylated, probably to asymmetric dimethylarginine.
SIMILARITY: SwissProt: P17844 ## Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily. & Contains 1 helicase ATP-binding domain. & Contains 1 helicase C-terminal domain.
SIZE: 614 amino acids; 69148 Da
SUBUNIT: Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRPA1, HNRPA2B1, HNRPA3, HNRPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRPR, HNRPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SFRS1, SKIV2L2, SNRPA1, SNRPB, SNRPB2, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, SNW1, SRRM1, SRRM2, SYF2, SYNCRIP, TFIP11, THOC4, U2AF1, WDR57, XAB2 and ZCCHC8.
SUBCELLULAR LOCATION: Nucleus, nucleolus.
PTM: Arg-502 is dimethylated, probably to asymmetric dimethylarginine.
SIMILARITY: SwissProt: P17844 ## Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily. & Contains 1 helicase ATP-binding domain. & Contains 1 helicase C-terminal domain.
Qty/Pk:
200 µg
Brand Family:
Upstate
Format:
Purified
Host:
Mouse
Product Name:
Anti-p68, clone PAb204
Antibody Type:
Monoclonal Antibody