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WB: Untreated (Lanes 1,3 and 5), sodium butyrate (Lane 2) or colcemid treated (Lane 4) and recombinant Histone H4 (lane 6) were probed with anti-Hist...
Epitope mapping (using Luminex®)
1:250 to 1:4,000 serial dilutions of anti-Histone H4, pan were incubated with a cocktail of microspheres conjuga...
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Anti-Histone H4, pan, clone 62-141-13
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, Ch, Vrt, Yeast (S. cerevisiae) | Chromatin Immunoprecipitation (ChIP), WB | Rabbit | Purified | Monoclonal Antibody |
Description:
Anti-Histone H4, pan, clone 62-141-13
Trade Name:
Upstate (Millipore)
Product Overview:
This antibody recognizes histone H4 independant of any post-translational modifications.
Specificity:
Recognizes Histone H4, Mr 10 kDa.
Molecular Weight:
10 kDa
Epitope:
a.a. 25-28
Immunogen:
KLH-conjugated, synthetic peptide corresponding to amino acids 17-28 of Histone H4. Epitope has been mapped to residues 25-28.
Isotype:
IgG
Background Information:
Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure.
Species Reactivity:
- Human
- Chicken
- Vertebrates
- Yeast (S. cerevisiae)
Species Reactivity Note:
Human, chicken and yeast. Broad species cross-reactivity expected based on sequence homology.
Application Notes:
Epitope Mapping (using Luminex®):
1:250-1:4,000 serial dilutions of a previous lot were incubated with histone peptides containing various unmodified peptides spanning the amino-terminus of Histone H4 conjugated to Luminex® microspheres. The antibody recognized both modified and unmodified peptides (See Figure B Page 2).
Chromatin Immunoprecipitation:
An independent laboratory used a previous lot of this antibody in chromatin immunoprecipitation (Issac, C., 2006).
1:250-1:4,000 serial dilutions of a previous lot were incubated with histone peptides containing various unmodified peptides spanning the amino-terminus of Histone H4 conjugated to Luminex® microspheres. The antibody recognized both modified and unmodified peptides (See Figure B Page 2).
Chromatin Immunoprecipitation:
An independent laboratory used a previous lot of this antibody in chromatin immunoprecipitation (Issac, C., 2006).
Control:
Acid extracts from both untreated HeLa cells and cells treated with sodium butyrate (hyper-acetylated) or colcemid (hyper-phosphorylated).
Quality Assurance:
Routinely evaluated by western blot.
Western Blot Analysis:
A 1:30,000-1:100,000 dilution of this lot detected unmodified recombinant Histone H4 protein (Catalog # 14-412) and Histone H4 in acid extracts from both untreated HeLa cells and cells treated with sodium butyrate (hyper-acetylated) or colcemid (hyper-phosphorylated)
Western Blot Analysis:
A 1:30,000-1:100,000 dilution of this lot detected unmodified recombinant Histone H4 protein (Catalog # 14-412) and Histone H4 in acid extracts from both untreated HeLa cells and cells treated with sodium butyrate (hyper-acetylated) or colcemid (hyper-phosphorylated)
Purification Method:
Protein A purfied
Presentation:
Purified rabbit monoclonal IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- HIST1H4A H4/J HIST1H4F HIST1H4L H4FN H4FA H4FH
- HIST1H4H
- H4F2
- H4FM
- H4/A
- H4FK
- H4FG
- HIST2H4
- H4FB
- H4/K
- HIST1H4I
- H4/N
- HIST1H4B
- H4FE
- H4/M
- H4/a
- HIST1H4E
- HIST4H4
- HIST2H4A
- H4FC
- H4FI
- H4/H
- HIST1H4C
- H4/G
- HIST1H4K
- H4FJ
- H4FD
- H4/I
- H4/B
- H4/D
- H4/C
- HIST1H4J
- HIST1H4D
- H4/E
Alternate Names:
- H4
- Histone H4
- H4 histone family, member A
- histone 1, H4a
- histone cluster 1, H4a
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Chromatin Immunoprecipitation (ChIP)
- Western Blotting
Entrez Gene Summary:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
UniProt Summary:
FUNCTION: SwissProt: P62805 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
SIZE: 103 amino acids; 11367 Da
SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
SUBCELLULAR LOCATION: Nucleus.
PTM: Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage (By similarity). & Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
SIMILARITY: SwissProt: P62805 ## Belongs to the histone H4 family.
SIZE: 103 amino acids; 11367 Da
SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
SUBCELLULAR LOCATION: Nucleus.
PTM: Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage (By similarity). & Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
SIMILARITY: SwissProt: P62805 ## Belongs to the histone H4 family.
Brand Family:
Upstate
Product Name:
Anti-Histone H4, pan, clone 62-141-13
Concentration:
Variable
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 µL
Format:
Purified
Host:
Rabbit