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Ordering Information

  • : 05-904
  • : 200 µg
  • : Please refer to the Certificate of Analysis for the lot-specific concentration.

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Western Blot Analysis
Lysates from untransfected (lane 1) and transfected (lane 2) HEK293 cells were resolved by electrophoresis, transferred to ...

WB; Lysates from untransfected (lane 1) and transfected (lane 2) HEK293 cells were probed with Anti-HA Tag (0.5μg/ml).

Anti-HA Tag Antibody

Recommended Replacement for: 05-904 is a recommended replacement for 07-221
Species Reactivity Key Applications Host Format Antibody Type
Vrt ICC, IP, WB Mouse Purified Monoclonal Antibody
Description:
Anti-HA Tag Antibody
Replaces:
05-904 is a recommended replacement for 07-221
Promotional Text:
Trade Name:
Upstate (Millipore)
Product Overview:
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
Specificity:
Recognizes HA tagged proteins overexpressed in cells, including both amino or carboxy-termini of targeted proteins in transfected mammalian cells.
Molecular Weight:
Various
Immunogen:
KLH-conjugated, synthetic peptide corresponding to amino acids 98-106 of influenza hemaglutinin.
Isotype:
Background Information:
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
Species Reactivity:
Vertebrates
Species Reactivity Note:
Most common vertebrate species tested. Reactivity with other species has not been confirmed.
Application Notes:
Immunoprecipitation:
5 μg/mL of his antibody has been reported by an independent laboratory to immunoprecipitate recombinant HA-tagged fusion protein.

Immunocytochemistry:
5 μg/mL of this antibody has been reported by an independent laboratory to immunostain HA-tagged fusion protein.
Control:
RIPA lysates from HEK293 cells.
Quality Assurance:
Routinely evaluated by western blot of recombinant protein containing the HA-tag at the amino terminus in RIPA lysates from HEK293 cells.

Western Blot Analysis:
0.5-2 μg/mL of this lot detected a recombinant protein containing the HA-tag at the amino terminus in RIPA lysates from HEK293 cells.
Purification Method:
Protein A purfied
Presentation:
Purified mouse monoclonal IgG3 in buffer containing PBS, pH 7.4 containing 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
  • Immunocytochemistry
  • Immunoprecipitation
  • Western Blotting
Brand Family:
Upstate
Product Name:
Anti-HA Tag Antibody
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Monoclonal Antibody
Qty/Pk:
200 µg
Format:
Purified
Host:
Mouse
Applications:
Detect HA Tag using this Anti-HA Tag Antibody validated for use in IC, IP & WB.

Product Resources

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