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Ordering Information

  • : 05-939
  • : 100 µL
  • : Please refer to the Certificate of Analysis for the lot-specific concentration.

Product Images

HeLa cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-LSD1/BHC110 (1:2000 dilution). Arrow indicates L...

Anti-LSD1/BHC110 Antibody

Recommended Replacement for: 06-574 is a recommended replacement for CBL770
Species Reactivity Key Applications Host Format Antibody Type
H, M IF, IHC, IP, WB, ChIP Mouse Ascites Monoclonal Antibody
Description:
Anti-LSD1/BHC110 Antibody
Replaces:
06-574 is a recommended replacement for CBL770
Promotional Text:
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes LSD1, Mr 110 kDa.
Molecular Weight:
110 kDa
Immunogen:
GST fusion protein corresponding to residues 23-138 of human LSD1.
Background Information:
Histone demethylase that specifically demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and tri-methylted 'Lys-4' of histone H3. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 'Lys-4' on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. May also demethylate 'Lys-9' of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes.
Species Reactivity:
  • Human
  • Mouse
Species Reactivity Note:
Human and mouse.
Application Notes:
Chromatin Immunoprecipitation:
This antibody has been reported by an independent laboratory to immunoprecipitate LSD1 from chromatin.

Immunoprecipitation:
Recommended.

Immunohistochemistry:
This antibody has been reported by an independent laboratory to detect LSD1 using paraffin-embedded tissues. (Metzger, E., 2005)

Immunofluorescence:
Recommended.
Control:
HeLa cell lysate.
Quality Assurance:
Routinely evaluated by western blot.

Western Blot Analysis:
1:500-1:2000 dilution of this lot detected LSD1 in RIPA lysates from HeLa cells.
Purification Method:
Unpurified
Presentation:
Mouse monoclonal in buffer containing 0.05% sodium azide and 30% glycerol.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
  • AOF2
  • KIAA0601
  • BHC110
  • LSD1
Alternate Names:
  • BRAF35-HDAC complex protein BHC110
  • FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit
  • Flavin-containing amine oxidase domain-containing protein 2
  • amine oxidase (flavin containing) domain 2
  • lysine (K)-specific demethylase 1
  • lysine-specific histone demethylase 1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
  • Immunofluorescence
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blotting
  • Chromatin Immunoprecipitation (ChIP)
Entrez Gene Summary:
This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternate splicing has been observed; however, not all transcript variants have been fully characterized.
UniProt Summary:
FUNCTION: SwissProt: O60341 # Histone demethylase that specifically demethylates 'Lys- 4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and tri- methylated 'Lys-4' of histone H3. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 'Lys-4' on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. May also demethylate 'Lys- 9' of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes.
COFACTOR: FAD.
SIZE: 852 amino acids; 92903 Da
SUBUNIT: Component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B/BRAF35, AOF2/LSD1, RCOR1/CoREST and PHF21A/BHC80. The BHC complex may also contain ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. In the complex, RCOR1/CoREST strongly enhances the demethylase activity and protects it from the proteasome while PHF21A/BHC80 inhibits the demethylase activity. Interacts with the androgen receptor (AR).
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Ubiquitously expressed.
DOMAIN: SwissProt: O60341 The SWIRN domain may act as an anchor site for a histone tail.
SIMILARITY: Belongs to the flavin monoamine oxidase family. & Contains 1 SWIRM domain.
Brand Family:
Upstate
Product Name:
Anti-LSD1/BHC110 Antibody
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 µL
Format:
Ascites
Host:
Mouse
Applications:
Anti-LSD1/BHC110 Antibody is a Mouse Monoclonal Antibody for detection of LSD1/BHC110 also known as BRAF35-HDAC complex protein BHC110, lysine (K)-specific demethylase 1 & has been validated in ChIP, IF, IHC, IP & WB.

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