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WB: EGF-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Phosphotyrosine (2µg/ml). Prot...
Anti-Phosphotyrosine Antibody, clone PY20 | 05-947
Recommended Replacement for: 05-947 is a recommended replacement for MAB3080| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Vrt | ELISA, IP, WB | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-Phosphotyrosine Antibody, clone PY20 | 05-947
Replaces:
05-947 is a recommended replacement for MAB3080
Promotional Text:
Trade Name:
Upstate (Millipore)
Specificity:
Tyrosine-phosphorylated proteins from all species.
Molecular Weight:
Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
Immunogen:
Phosphotyrosine conjugated to carrier protein.
Modifications:
Phosphorylation
Clone:
PY20
Isotype:
IgG2b
Background Information:
Tyrosine phosphorylation represents a major mechanism for the activation of signal transduction pathways in response to growth factors, hormones and cytokines. There are approximately 100 tyrosine kinases that are both receptor tyrsosine kinase receptors for various growth factors and cytokines. The extent and duration of tyrosine phosphorylation are tightly regulated by the 107 protein tyrosine phosphatases. Tyrosine phosphorylation occurs on residues on either other kinases (Ser/Thr or Tyr) or on substrates. In the case of kinases, this phosphorylation often causes the enzyme to change activation states and/or it creates binding sites for SH2 domain. Mutation or misregulation of protein tyrosine kinases is a cause or consequence of many disease states. It has been estimated that 20% of oncogenes so far described are tyrosine kinases. With such clear links between aberrant protein tyrosine kinase activity and disease, it is no surprise that much of the early effort in kinase drug discovery was focused on these enzymes.
Species Reactivity:
Vertebrates
Species Reactivity Note:
Most common vertebrate species tested. Reactivity with other species has not been confirmed.
Application Notes:
Immunoprecipitation:
Recommended by an independent laboratory to immunoprecipitate tyrosine phosphorylated proteins.
ELISA:
Recommended by an independent laboratory.
Recommended by an independent laboratory to immunoprecipitate tyrosine phosphorylated proteins.
ELISA:
Recommended by an independent laboratory.
Control:
EGF-stimulated A431 cell lysate.
Included Positive Antigen Control: Catalog # 12-302, EGF-stimulated A431 cell lysate is provided as a free positive antigen control for western immunoblotting. Aliquot as desired, refreeze immediately, and store at -20°C. The lysate is stable for 6 months at -20°C. Before use, add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for immunoblot analysis.
Quality Assurance:
Routinely evaluated by western blot with RIPA lysates EGF-treated human A431 carcinoma cells.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected tyrosine-phosphorylated proteins in RIPA lysates EGFtreated human A431 carcinoma cells.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected tyrosine-phosphorylated proteins in RIPA lysates EGFtreated human A431 carcinoma cells.
Purification Method:
Protein A purfied
Presentation:
0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4. Liquid at 2-8°C.
Storage Conditions:
Stable for 1 year at 2-8°C from date of recipt.
NOTE: DO NOT FREEZE.
For maximum recovery of the product, centrifuge the original vial prior to removing the cap. If the product has accidentally been frozen and thawed, spin it at 13,000 x g for 10 minutes at 2-8°C. Save the supernatant for application.
For maximum recovery of the product, centrifuge the original vial prior to removing the cap. If the product has accidentally been frozen and thawed, spin it at 13,000 x g for 10 minutes at 2-8°C. Save the supernatant for application.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- ELISA
- Immunoprecipitation
- Western Blotting
Brand Family:
Upstate
Product Name:
Anti-Phosphotyrosine Antibody, clone PY20 | 05-947
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody
Qty/Pk:
1 mg
Format:
Purified
Host:
Mouse