BrasilInformações para Pedidos
- : 06-009
- : 100 μg
- : 0.25 mg/mL
Product Images
Western Blot Analysis:
Representative lot data.
RAW264.7 lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-TL...
Western Blot Analysis: Representative lot data.
Rat spleen (lane 1), rat thymus (lane 2) and RAW264.7 (lane 3) lysates were resolved by electroph...
Anti-TLR3, C-terminus
| Reatividade à Espécie | Principais aplicações | Hospedeiro | Formato | Tipo de Anticorpo |
|---|---|---|---|---|
| M, R | WB | Rabbit | Purified | Polyclonal Antibody |
Description:
Anti-TLR3, C-terminus
Specificity:
This antibody recognizes the N-terminal domain of TLR3.
Molecular Weight:
~103 kDa
Epitope:
C-terminus
Immunogen:
KLH-conjugated synthetic peptide corres-ponding to the C-terminal region of TLR3.
Background Information:
The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. TLR1 recognizes peptidoglycan and (triacyl) lipoproteins on pathogens, especially gram-positive bacteria. TLR1 is found on the surface of macrophages and neutrophils.
Species Reactivity:
- Mouse
- Rat
Control:
Rat thymus, rat spleen or RAW264.7 lysates.
Quality Assurance:
Evaluated by western blot on RAW264.7 lysate.
Western Blot Analysis:
1 μg/mL of this antibody was used to detect TLR3 in RAW264.7 lysate.
Western Blot Analysis:
1 μg/mL of this antibody was used to detect TLR3 in RAW264.7 lysate.
Purification Method:
Protein G purfied
Presentation:
Purified rabbit polyclonal in buffer containing PBS and 0.09% sodium azide.
Storage Conditions:
Stable for 6 months at 2-8ºC from date of receipt. For long term storage, store at -20ºC.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
CD283
Alternate Names:
- CD283 antigen
- toll-like receptor 3
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Western Blotting
Entrez Gene Summary:
The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor is most abundantly expressed in placenta and pancreas, and is restricted to the dendritic subpopulation of the leukocytes. It recognizes dsRNA associated with viral infection, and induces the activation of NF-kappaB and the production of type I interferons. It may thus play a role in host defense against viruses. Use of alternative polyadenylation sites to generate different length transcripts has been noted for this gene. [provided by RefSeq].
UniProt Summary:
FUNCTION: Participates in the innate immune response to microbial agents. Mediates the innate immune response to ds-RNA, a sign of viral infection. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10
SUBUNIT STRUCTURE: Binds MYD88 via their respective TIR domains By similarity. Interacts with TICAM1. Homodimer formation is triggered by ligand binding and is required for TLR3 signaling. Binding of ds-RNA is required for the interaction with SRC.
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass type I membrane protein. Endosome membrane. Ref.6
TISSUE SPECIFICITY: Expressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes, including monocyte precursors.
PTM: Heavily N-glycosylated, except on that part of the surface of the ectodomain that is involved in ligand binding. Ref.10 Ref.11
SIMILARITY: Belongs to the Toll-like receptor family.
Contains 22 LRR (leucine-rich) repeats.
Contains 1 TIR domain.
SUBUNIT STRUCTURE: Binds MYD88 via their respective TIR domains By similarity. Interacts with TICAM1. Homodimer formation is triggered by ligand binding and is required for TLR3 signaling. Binding of ds-RNA is required for the interaction with SRC.
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass type I membrane protein. Endosome membrane. Ref.6
TISSUE SPECIFICITY: Expressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes, including monocyte precursors.
PTM: Heavily N-glycosylated, except on that part of the surface of the ectodomain that is involved in ligand binding. Ref.10 Ref.11
SIMILARITY: Belongs to the Toll-like receptor family.
Contains 22 LRR (leucine-rich) repeats.
Contains 1 TIR domain.
Product Name:
Anti-TLR3, C-terminus
Concentration:
0.25 mg/mL
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 μg
Format:
Purified
Host:
Rabbit