Ordering Information
- : 06-1038
- : 100 μg
- : 0.5 mg/mL
Product Images
Western Blot (SNAP ID) Analysis:
Representative lot data.
U2OS lysate treated with immunoprecipitated anti-Unc84B/SUN2 was resolved by ele...
Western Blot Analysis:
Representative lot data.
U2OS lysate treated with immunoprecipitated anti-Unc84B/SUN2 was resolved by electrophores...
Anti-Unc84B/SUN2 Antibody
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, Ca, Eq | IP, WB | Rabbit | Affinity Purified | Polyclonal Antibody |
Description:
Anti-Unc84B/SUN2 Antibody | 06-1038
Promotional Text:
Specificity:
This antibody recognizes Unc84B/SUN2 at the N-terminus.
Molecular Weight:
~80 kDa
Epitope:
N-terminus
Immunogen:
KLH-conjugated linear peptide corresponding to human Unc84B/SUN2 at and around the N-terminus.
Isotype:
IgG
Background Information:
UNC84B (Protein UNC-84 homolog B or SUN2), is a single-pass nuclear envelope transmembrane protein responsible for nuclear migrations that are essential for development. UNC-84B has a predicted transmembrane domain and a C-terminal region with similarity to the S. pombe spindle pole body protein Sad1. UNC-84B interacts with RAB5A and is widely expressed, and highly expressed in the heart, lungs and muscles. UNC-84 may function to facilitate a nuclear-centrosomal interaction required for nuclear migration and anchorage.
Species Reactivity:
- Human
- Canine
- Horse
Species Reactivity Note:
Proven to react with human. Predicted to react with dog and horse based on sequence homology. Mouse and rat (87%).
Application Notes:
Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated Unc84B/SUN2 from 500 µg of U2OS cell lysate.
Control:
U2OS cell lysate.
Quality Assurance:
Evaluated by Western Blotting in U2OS cell lysate.
Western Blot (SNAP ID) Analysis: 1:250 dilution of this antibody detected Unc84B/SUN2 on 10 µg of U2OS cell lysate.
Western Blot (SNAP ID) Analysis: 1:250 dilution of this antibody detected Unc84B/SUN2 on 10 µg of U2OS cell lysate.
Purification Method:
Affinity Purfied
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- FRIGG
- UNC84B
- RAB5IP
- Rab5IP
- SUN2
Alternate Names:
- Rab5-interacting protein
- Sad1 unc-84 domain protein 2
- Sad1/unc-84 protein-like 2
- nuclear envelope protein
- unc-84 homolog B
- unc-84 homolog B (C. elegans)
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Immunoprecipitation
- Western Blotting
UniProt Summary:
SUBUNIT STRUCTURE: Interacts with RAB5A.
SUBCELLULAR LOCATION: Nucleus membrane; Single-pass membrane protein. Endosome membrane; Single-pass membrane protein Probable.
TISSUE SPECIFICTY: Widely expressed. Highly expressed in heart, lung and muscle. Weakly expressed in fetal heart. Slightly overexpressed in some heart tissues form patients with congenital heart defects.
DOMAIN: The Unc84 domain may play a role in the nuclear anchoring and/or migration.
SEQUENCE SIMILARITIES: Contains 1 Unc84 (SUN) domain.
Caution It is uncertain whether Met-1 or Met-50 is the initiator.
SUBCELLULAR LOCATION: Nucleus membrane; Single-pass membrane protein. Endosome membrane; Single-pass membrane protein Probable.
TISSUE SPECIFICTY: Widely expressed. Highly expressed in heart, lung and muscle. Weakly expressed in fetal heart. Slightly overexpressed in some heart tissues form patients with congenital heart defects.
DOMAIN: The Unc84 domain may play a role in the nuclear anchoring and/or migration.
SEQUENCE SIMILARITIES: Contains 1 Unc84 (SUN) domain.
Caution It is uncertain whether Met-1 or Met-50 is the initiator.
Product Name:
Anti-Unc84B/SUN2
Concentration:
0.5 mg/mL
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 μg
Format:
Affinity Purified
Host:
Rabbit