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Ordering Information

  • : 06-1047
  • : 100 µg
  • : Please refer to the Certificate of Analysis for the lot-specific concentration.

Product Images

Immunocytochemistry:
Representative lot data.
Confocal fluorescent analysis of PC3 cells using Anti-IRF4. Actin filaments have been labeled ...

Western Blot Analysis:
Representative lot data.
Mouse brain lysate was resolved by electrophoresis, transferred to PVDF and probed with anti...

Anti-IRF4, N-terminus

Species Reactivity Key Applications Host Format Antibody Type
H, M, Eq, R WB, ICC Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-IRF4 Antibody, NT
Promotional Text:
Specificity:
This antibody recognizes the N-terminal region of IRF4 at and around amino acids 177-194.
Molecular Weight:
~52 kDa
Epitope:
N-Terminus
Immunogen:
KLH conjugated linear peptide corresponding to the N-terminal region of IRF4.
Background Information:
Interferon regulatory factor 4, also known as IRF4, belongs to the IRF family. IRF4 encodes a 450 amino acid polypeptide with a predicted molecular weight of 51.6 kDa (Grossman 1996). The IRF4 gene is expressed in the spleen, lymphocytes and melanocytes, and is induced in to T cells after cross linking with the T cell receptor. IRF4 is a transcriptional activator that binds to the interferon stimulated response element (ISRE) of the MHC class I promoter. IRF4 is essential for mature T and B lymphocyte function. IRF4 interacts with MYD88 to prevent the interaction of IRF5, resulting in negative regulation of Toll Like Receptor (TLR) signaling. High levels of IRF4 have been associated with Epstein Barr Virus (EBV) and may be critical to EBV transformation. Further oncogenic potential has been associated with the involvement of IRF4 with hair color and pigmentation of the skin, which may cause multiple myeloma.
Species Reactivity:
  • Human
  • Mouse
  • Horse
  • Rat
Species Reactivity Note:
Tested to react with human and mouse. Predicted to react with rat and horse based on sequence homology.
Application Notes:
Immunocytochemistry: 1:500 dilution of a previous lot of this antibody was used on immunocytochemistry in PC3 cells.
Control:
Mouse brain lysate.
Quality Assurance:
Evaluated by western blot on mouse brain lysate.
Western Blot Analysis: 0.5 μg/mL of this antibody detected IRF4 in 10 μg of mouse brain lysate.
Purification Method:
Affinity Purfied
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8ºC from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
  • IRF-4
  • LSIRF
  • MUM1
  • NF-EM5
Alternate Names:
  • Lymphocyte-specific interferon regulatory factor
  • Multiple myeloma oncogene
  • interferon regulatory factor 4
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
  • Western Blotting
  • Immunocytochemistry
UniProt Summary:
Function: Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells.
Subunit structure: Interacts with SPIB and DEF6. Ref.5 Ref.6
Subcellular location: Nucleus.
Tissue specificity: Lymphoid cells.
Induction: Not induced by interferons.
Involvement in disease: A chromosomal aberration involving IRF4 may be a cause of multiple myeloma [MIM:254500]. Translocation t(6;14)(p25;q32) with the IgH locus.
Sequence similarities: Belongs to the IRF family.
Contains 1 tryptophan pentad repeat DNA-binding domain.

Product Name:
Anti-IRF4, N-terminus
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 µg
Format:
Affinity Purified
Host:
Rabbit
Applications:
Anti-IRF4 Antibody, N-terminus detects level of IRF4 & has been published & validated for use in WB & IC.

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