Ordering Information
- : 06-1050
- : 100 μg
- : 1.0 mg/mL
Product Images
Immunohistochemistry Analysis:
Representative lot data.
Paraffin-embedded colorectal cancer (Fig. 1) and multiform glioblastoma (Fig. 2 & ...
Western Blotting Analysis:
Representative lot data.
SW620 cell lysate was probed with Anti-Axin-2 (0.5 µg/mL).
Proteins were visual...
Anti-Axin-2
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IH(P) | Rabbit | Affinity Purified | Polyclonal Antibody |
Description:
Anti-Axin-2 Antibody | 06-1050
Promotional Text:
Molecular Weight:
~85 kDa observed
Immunogen:
KLH-conjugated linear peptide corresponding to human Axin-2.
Background Information:
Axis inhibition protein 2 (Axin-2) has been linked to selective tooth angenesis. It has been found in human thymus, prostate, testis, small intestine, ovarian tissue and in the colon, at a lower expression rate. Axin-2 is involved during skull morphogenesis and missing Axin-2 can cause abnormal skull morphology. Variation in Axin-2 has been associated with accelerated chondrocyte proliferation.
Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react with Human.
Application Notes:
Immunohistochemistry Analysis: 1:500 dilution from a representative lot detected Axin-2 in colorectal cancer and multiform glioblastoma tissues.
Control:
SW620 cell lysate
Quality Assurance:
Evaluated by Western Blot in SW620 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Axin-2 on 10 µg of SW620 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Axin-2 on 10 µg of SW620 cell lysate.
Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
AXIN2
Alternate Names:
- Axin-2
- Axin-like protein
- Axil
- Axis inhibition protein 2
- Conductin
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Western Blotting
- Immunohistochemistry (Paraffin)
Entrez Gene Summary:
The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway, like its rodent homologs, mouse conductin/rat axil. In mouse, conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and conductin, which leads to the degradation of beta-catenin. Apparently, the deregulation of beta-catenin is an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair. [provided by RefSeq].
UniProt Summary:
FUNCTION: Inhibitor of the Wnt signaling pathway. Down-regulates beta-catenin. Probably facilitate the phosphorylation of beta-catenin and APC by GSK3B (By similarity).
SUBUNIT STRUCTURE: Interacts with glycogen synthase kinase-3 beta (GSK3B) and beta-catenin. The interaction between axin and beta-catenin occurs via the armadillo repeats contained in beta-catenin (By similarity). Interacts with SMAD7 and RNF111. Interacts with ANKRD6.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Expressed in brain and lymphoblast.
PTM: Probably phosphorylated by GSK3B and dephosphorylated by PP2A (By similarity).
INVOLVEMENT IN DISEASE: Defects in AXIN2 are involved in colorectal cancer (CRC) [MIM:114500]. They appear to be specifically associated with defective mismatch repair.
Defects in AXIN2 are the cause of oligodontia-colorectal cancer syndrome (ODCRCS) [MIM:608615]. Affected individuals manifest severe tooth agenesis and colorectal cancer or precancerous lesions of variable types.
SEQUENCE SIMILARITIES: Contains 1 DIX domain.
Contains 1 RGS domain.
SUBUNIT STRUCTURE: Interacts with glycogen synthase kinase-3 beta (GSK3B) and beta-catenin. The interaction between axin and beta-catenin occurs via the armadillo repeats contained in beta-catenin (By similarity). Interacts with SMAD7 and RNF111. Interacts with ANKRD6.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Expressed in brain and lymphoblast.
PTM: Probably phosphorylated by GSK3B and dephosphorylated by PP2A (By similarity).
INVOLVEMENT IN DISEASE: Defects in AXIN2 are involved in colorectal cancer (CRC) [MIM:114500]. They appear to be specifically associated with defective mismatch repair.
Defects in AXIN2 are the cause of oligodontia-colorectal cancer syndrome (ODCRCS) [MIM:608615]. Affected individuals manifest severe tooth agenesis and colorectal cancer or precancerous lesions of variable types.
SEQUENCE SIMILARITIES: Contains 1 DIX domain.
Contains 1 RGS domain.
Product Name:
Anti-Axin-2
Concentration:
1.0 mg/mL
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 μg
Format:
Affinity Purified
Host:
Rabbit