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WB: A431 cell lysate probed with anti-Cyclin D (1μg/ml).
Anti-Cyclin D
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | IC, IP, WB | Rabbit | Purified | Polyclonal Antibody |
Description:
Anti-Cyclin D
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Cyclin D1 and D2; no cross-reactivity with Cyclin A or B.
Molecular Weight:
Cyclin D1 (36 kDa) and Cyclin D2 (34 kDa)
Immunogen:
peptide corresponding the 11 C-terminal amino acids. residues 285-295, of human Cyclin D1 (CTPTDVRDVDI)
Isotype:
IgG
Background Information:
Progression through G1 phase and transition from G1 to S phase of the cell division cycle is controlled by activation of a distinct series of serine/threonine kinase complexes which comprise of a cyclin regulatory subunit and a cyclin dependent kinase (cdk). D type cyclins are induced earlier than cyclin E in G1 and can form complexes with cdk2,-4,-5, and -6. Cyclin D1 is a nuclear protein during G1 phase and disappears from the nucleus in S-phase. Among the key cell cycle regulators, cyclin D1 has been implicated most strongly as a proto-oncogene in several human tumor types, including breast carcinomas.
Species Reactivity:
- Human
- Mouse
- Rat
Control:
Positive Control Included: 3T3 Cell Lysate (12-305)
Quality Assurance:
routinely evaluated by immunoblot on RIPA lysates of mouse 3T3/A31 fibroblasts or EGF stimulated human A431 cells
Purification Method:
Protein A purfied
Presentation:
Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
Storage Conditions:
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- CCND1
- D11S287E
- U21B31
- PRAD1
- BCL1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.
Key Applications:
- Immunocytochemistry
- Immunoprecipitation
- Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P24385 # Essential for the control of the cell cycle at the G1/S (start) transition.
SIZE: 295 amino acids; 33729 Da
SUBUNIT: Interacts with the CDK4 and CDK6 protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex.
DISEASE: SwissProt: P24385 # A chromosomal aberration involving CCND1 may be a cause of B-lymphocytic malignancy, particularly mantle-cell lymphoma (MCL). Translocation t(11;14)(q13;q32) with immunoglobulin gene regions. Activation of CCND1 may be oncogenic by directly altering progression through the cell cycle. & A chromosomal aberration involving CCND1 may be a cause of parathyroid adenomas [MIM:168461]. Translocation t(11;11)(q13;p15) with the parathyroid hormone (PTH) enhancer. & A chromosomal aberration involving CCND1 may be a cause of multiple myeloma [MIM:254500]. Translocation t(11;14)(q13;q32) with the IgH locus.
SIMILARITY: SwissProt: P24385 ## Belongs to the cyclin family. Cyclin D subfamily.
SIZE: 295 amino acids; 33729 Da
SUBUNIT: Interacts with the CDK4 and CDK6 protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex.
DISEASE: SwissProt: P24385 # A chromosomal aberration involving CCND1 may be a cause of B-lymphocytic malignancy, particularly mantle-cell lymphoma (MCL). Translocation t(11;14)(q13;q32) with immunoglobulin gene regions. Activation of CCND1 may be oncogenic by directly altering progression through the cell cycle. & A chromosomal aberration involving CCND1 may be a cause of parathyroid adenomas [MIM:168461]. Translocation t(11;11)(q13;p15) with the parathyroid hormone (PTH) enhancer. & A chromosomal aberration involving CCND1 may be a cause of multiple myeloma [MIM:254500]. Translocation t(11;14)(q13;q32) with the IgH locus.
SIMILARITY: SwissProt: P24385 ## Belongs to the cyclin family. Cyclin D subfamily.
Qty/Pk:
200 μg
Brand Family:
Upstate
Format:
Purified
Host:
Rabbit
Product Name:
Anti-Cyclin D
Antibody Type:
Polyclonal Antibody