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Anti-Cyclin A
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R, Sh | IP, WB, IC | Rabbit | Purified | Polyclonal Antibody |
Description:
Anti-Cyclin A
Trade Name:
Upstate (Millipore)
Specificity:
p58cyclin A; does not cross-react with cyclin B or cyclin D2, or 3
Molecular Weight:
58kDa
Immunogen:
peptide corresponding amino acid residues 417-432 (C-KHGVSLLNPPETL-N-L)
Isotype:
IgG
Background Information:
The cell cycle is regulated by the interplay of many molecules. Key among these are the cyclins which are expressed and then degraded in a concerted fashion to drive the stages of the cell cycle. Cyclins combine with cyclin dependent kinases (cdks) to form activated kinases that phosphorylate targets leading to cell cycle regulation. A breakdown in the regulation of this cycle can lead to out of control growth and contribute to tumor formation. Defects in many of the molecules that regulate the cell cycle have been implicated in cancer. Cyclin A is a member of the Cyclin A family that can bind and activate Cdc2 and Cdk2 and is involved in the control of mitosis and S phase. During S phase, cyclin A forms a complex with Cdk2, the pRB-related protein p107 and E2F.
Species Reactivity:
- Human
- Mouse
- Rat
- Sheep
Control:
Daudi cells
Quality Assurance:
routinely evaluated by immunoblot on HeLa nuclear extract
Purification Method:
Protein G chromatography
Presentation:
Lyophilized
Storage Conditions:
2 years at -20°C
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- CCNA2
- Cyclin-A2
- CCN1
- Cyclin-A
- CCNA
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.
Key Applications:
- Immunoprecipitation
- Western Blotting
- Immunocytochemistry
UniProt Summary:
FUNCTION: SwissProt: P20248 # Essential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions.
SIZE: 432 amino acids; 48537 Da
SUBUNIT: Interacts with the CDK2 and CDC2 protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex.
DEVELOPMENTAL STAGE: Accumulates steadily during G2 and is abruptly destroyed at mitosis.
SIMILARITY: SwissProt: P20248 ## Belongs to the cyclin family. Cyclin AB subfamily.
SIZE: 432 amino acids; 48537 Da
SUBUNIT: Interacts with the CDK2 and CDC2 protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex.
DEVELOPMENTAL STAGE: Accumulates steadily during G2 and is abruptly destroyed at mitosis.
SIMILARITY: SwissProt: P20248 ## Belongs to the cyclin family. Cyclin AB subfamily.
Qty/Pk:
200 µg
Brand Family:
Upstate
Format:
Purified
Host:
Rabbit
Product Name:
Anti-Cyclin A
Antibody Type:
Polyclonal Antibody