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Ordering Information
- : 06-195
- : 125 μL
- : Variable
Product Images
Anti-PI3 Kinase, p85
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, Mk, R | IP, WB | Rabbit | Serum | Polyclonal Antibody |
Description:
Anti-PI3 Kinase, p85
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes p85 subunit of PI3 kinase.
Molecular Weight:
85 kDa
Immunogen:
Glutathione-S-Transferase fusion proteins containing 1) the N-terminal SH2 domain of rat PI3 kinase and 2) the full length 85kDa subunit of rat PI3 kinase
Isotype:
IgG
Background Information:
PI3 Kinase (Phosphatidylinositol 3-Kinase) is responsible for phosphorylation of the 3 position of the inositol ring of PI(4,5)P2, to generate PI(3,4,5)P3, a potent second messenger required for survival signaling, and insulin action. PI3 Kinase is a heterodimeric complex composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Tyrosine phosphorylation of growth factor receptors creates docking sites for binding of p85 (through its SH2 domains) on the receptors; p85 brings with it p110, which is then proximal to its phospho-lipid substrate on the membrane. PI3 Kinase is also activated by Ras, and by the β,γ subunits of heterotrimeric G-proteins. PI3 Kinase is inhibitable by wortmannin, a useful tool for the study of the PI3 Kinase signaling pathway.
Species Reactivity:
- Human
- Mouse
- Monkey
- Rat
Control:
Positive Control Included: Jurkat cell lysate (12-303)
Quality Assurance:
routinely evaluated by immunoblot on human Jurkat T cells, lysates from mouse NIH 3T3 fibroblasts, rat L6 myoblasts or EGF-stimulated human A431 cells
Purification Method:
Unpurified
Presentation:
Immunoaffinity Purified immunoglobulin in 0.2M Tris, 150mM NaCl, 45mM glycine, 0.05% sodium azide and 5mg/ml BSA before the addition of glycerol to 30%
Storage Conditions:
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
UniProt Number:
Gene Symbol:
- PIK3R1
- GRB1
- PI3K
- p85-ALPHA
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Immunoprecipitation
- Western Blotting
Qty/Pk:
125 μL
Brand Family:
Upstate
Format:
Serum
Host:
Rabbit
Product Name:
Anti-PI3 Kinase, p85
Concentration:
Variable
Antibody Type:
Polyclonal Antibody