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WB: HeLa nuclear extract was probed with anti-ATF-2 (2μg/ml).
Anti-ATF2
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | Sheep | Purified | Polyclonal Antibody |
Description:
Anti-ATF2
Trade Name:
Upstate (Millipore)
Specificity:
ATF2 protein, may cross-react with GST-fusion proteins; no cross-reactivity was observed for either ATF-1 or ATF-3.
Molecular Weight:
65kDa
Immunogen:
GST-fusion protein expressed in E. coli containing residues 95-211 of full length human ATF2 (Activating Transcription Factor-2, also called CRE-BP1)
Isotype:
IgG
Species Reactivity:
Human
Quality Assurance:
routinely evaluated by immunoblot on HeLa nuclear extract
Purification Method:
Protein G chromatography
Presentation:
0.1M Tris glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
Storage Conditions:
2 years at -20°C
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- ATF2
- MGC111558
- CREB2
- TREB7
- CRE-BP1
- CREBP1
- HB16
Components:
IP/Immunoblotting: use 2 micrograms to immunoprecipitate ATF-2 from HeLa nuclear extract (catalog #12-309); use 4 microgram/ml to detect by immunoblot
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. The protein forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription. The protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. Additional transcript variants have been identified but their biological validity has not been determined.
Key Applications:
Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P15336 # Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'- GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2-c-Jun complex.
SIZE: 487 amino acids; 52277 Da
SUBUNIT: Binds DNA as a dimer and can form a homodimer in the absence of DNA. Can form a heterodimer with JUN. Interacts with SMAD3 and SMAD4. Binds through its N-terminal region to UTF1 which acts as a coactivator of ATF2 transcriptional activity.
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Abundant expression seen in the brain.
PTM: Phosphorylation of Thr-51 and Thr-53 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK.
SIMILARITY: SwissProt: P15336 ## Belongs to the bZIP family. ATF subfamily. & Contains 1 bZIP domain. & Contains 1 C2H2-type zinc finger.
SIZE: 487 amino acids; 52277 Da
SUBUNIT: Binds DNA as a dimer and can form a homodimer in the absence of DNA. Can form a heterodimer with JUN. Interacts with SMAD3 and SMAD4. Binds through its N-terminal region to UTF1 which acts as a coactivator of ATF2 transcriptional activity.
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Abundant expression seen in the brain.
PTM: Phosphorylation of Thr-51 and Thr-53 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK.
SIMILARITY: SwissProt: P15336 ## Belongs to the bZIP family. ATF subfamily. & Contains 1 bZIP domain. & Contains 1 C2H2-type zinc finger.
Qty/Pk:
200 µg
Brand Family:
Upstate
Format:
Purified
Host:
Sheep
Product Name:
Anti-ATF2
Antibody Type:
Polyclonal Antibody