Ordering Information
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Western Blot Analysis
Representative blot from a previous lot.
HL-60 cell lysate was resolved by electrophoresis, transferred to nitrocellulos...
Anti-Bax, NT
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | IC, IH, IH(P), IP, WB | Rabbit | Purified | Polyclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: Q07814 # Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor Bcl-2 or its adenovirus homolog E1B 19k protein.| Q07812 # Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Induces the release of cytochrome c, activation of CASP3, and thereby apoptosis.
SIZE: 41 amino acids; 4678 Da
SUBUNIT: Interacts with HN.
SUBCELLULAR LOCATION: Cytoplasm ... see more »
SIZE: 41 amino acids; 4678 Da
SUBUNIT: Interacts with HN.
SUBCELLULAR LOCATION: Cytoplasm ... see more »
Entrez Gene Number:
Entrez Gene Summary
The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of
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Description:
Anti-Bax, NT
Background Information:
BAX (BCL2-associated X protein) belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. BAX protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene.
Alternate Names:
- BCL2-associated X protein
- apoptosis regulator BAX
Specificity:
Bax α p20 and β p23.
Key Applications:
- Immunocytochemistry
- Immunohistochemistry
- Immunohistochemistry (Paraffin)
- Immunoprecipitation
- Western Blotting
Application Notes:
Immunoprecipitation:
4 μg of previous lots immunoprecipitated Bax from human HL-60 and mouse ABE 8 1/2 lysates.
Immunocytochemistry:
10 μg/mL of previous lots detected Bax in mouse ABE 8 1/2 cells fixed in 1% paraformaldehyde, followed by permeabilization with 100% methanol for 60 seconds.33
Immunohistochemistry (Paraffin):
Paraffin embedded, well-differentiated ductal adenocarcinoma, stained with anti-Bax NT (10 μg/mL)
4 μg of previous lots immunoprecipitated Bax from human HL-60 and mouse ABE 8 1/2 lysates.
Immunocytochemistry:
10 μg/mL of previous lots detected Bax in mouse ABE 8 1/2 cells fixed in 1% paraformaldehyde, followed by permeabilization with 100% methanol for 60 seconds.33
Immunohistochemistry (Paraffin):
Paraffin embedded, well-differentiated ductal adenocarcinoma, stained with anti-Bax NT (10 μg/mL)
Species Reactivity:
- Human
- Mouse
Species Reactivity Note:
Human and mouse.
Isotype:
IgG
Immunogen:
Synthetic Peptide [MDGSGEQPRGGGPTSS EQIMK-C], corresponding to amino acid residues 1-21 of human Bax with a cysteine residue added on the C-terminus for conjugation to KLH.
Epitope:
a.a. 1-21
Molecular Weight:
23 kDa
Control:
Human heart tissue, human kidney tiussue, human placenta tissue, HepG2 cell lysate.
Presentation:
Purified rabbit IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Gene Symbol:
BAX
Quality Assurance:
Routinely evaluated by western blot on cell lysates from human HL-60 cells.
Western Blot Analysis: 0.5-2 μg/mL of this lot detected Bax in 20 μg of cell lysates from human HL-60 cells.
Western Blot Analysis: 0.5-2 μg/mL of this lot detected Bax in 20 μg of cell lysates from human HL-60 cells.
Antibody Category:
Apoptosis & Cancer
Antibody Sub-Category:
BCL2 & Inhibition
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Upstate (Millipore)
Format Code:
Pur
Concentration:
1 mg/mL
Antibody Type:
Polyclonal Antibody
Format:
Purified
Host:
Rabbit
Purification Method:
Protein G chromatography