Content Loading...
Content Loading...
Product Images
Western Blot Analysis
Representative blot from a previous lot.
HL-60 cell lysate was resolved by electrophoresis, transferred to nitrocellul...
Anti-Bax, NT
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | IC, IH, IH(P), IP, WB | Rabbit | Purified | Polyclonal Antibody |
Description:
Anti-Bax, NT
Trade Name:
Upstate (Millipore)
Specificity:
Bax α p20 and β p23.
Molecular Weight:
23 kDa
Epitope:
a.a. 1-21
Immunogen:
Synthetic Peptide [MDGSGEQPRGGGPTSS EQIMK-C], corresponding to amino acid residues 1-21 of human Bax with a cysteine residue added on the C-terminus for conjugation to KLH.
Isotype:
IgG
Background Information:
BAX (BCL2-associated X protein) belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. BAX protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene.
Species Reactivity:
- Human
- Mouse
Species Reactivity Note:
Human and mouse.
Application Notes:
Immunoprecipitation:
4 μg of previous lots immunoprecipitated Bax from human HL-60 and mouse ABE 8 1/2 lysates.
Immunocytochemistry:
10 μg/mL of previous lots detected Bax in mouse ABE 8 1/2 cells fixed in 1% paraformaldehyde, followed by permeabilization with 100% methanol for 60 seconds.33
Immunohistochemistry (Paraffin):
Paraffin embedded, well-differentiated ductal adenocarcinoma, stained with anti-Bax NT (10 μg/mL)
4 μg of previous lots immunoprecipitated Bax from human HL-60 and mouse ABE 8 1/2 lysates.
Immunocytochemistry:
10 μg/mL of previous lots detected Bax in mouse ABE 8 1/2 cells fixed in 1% paraformaldehyde, followed by permeabilization with 100% methanol for 60 seconds.33
Immunohistochemistry (Paraffin):
Paraffin embedded, well-differentiated ductal adenocarcinoma, stained with anti-Bax NT (10 μg/mL)
Control:
Human heart tissue, human kidney tiussue, human placenta tissue, HepG2 cell lysate.
Quality Assurance:
Routinely evaluated by western blot on cell lysates from human HL-60 cells.
Western Blot Analysis: 0.5-2 μg/mL of this lot detected Bax in 20 μg of cell lysates from human HL-60 cells.
Western Blot Analysis: 0.5-2 μg/mL of this lot detected Bax in 20 μg of cell lysates from human HL-60 cells.
Purification Method:
Protein G chromatography
Presentation:
Purified rabbit IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
BAX
Alternate Names:
- BCL2-associated X protein
- apoptosis regulator BAX
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Immunocytochemistry
- Immunohistochemistry
- Immunohistochemistry (Paraffin)
- Immunoprecipitation
- Western Blotting
Entrez Gene Summary:
The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene.
UniProt Summary:
FUNCTION: SwissProt: Q07814 # Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor Bcl-2 or its adenovirus homolog E1B 19k protein.| Q07812 # Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Induces the release of cytochrome c, activation of CASP3, and thereby apoptosis.
SIZE: 41 amino acids; 4678 Da
SUBUNIT: Interacts with HN.
SUBCELLULAR LOCATION: Cytoplasm.
DOMAIN: SwissProt: Q07815
DISEASE:SwissProt: Q07812 # Defects in BAX are found in some cell lines from hematopoietic malignancies as T-cell acute lymphoblastic leukemia, Burkitt lymphoma, and plasmacytoma.
SIMILARITY: SwissProt: Q07815 ## Belongs to the Bcl-2 family. | Q07814 # Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.# Belongs to the Bcl-2 family. | P55269 ## Belongs to the Bcl-2 family. | Q07812 # Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family (By similarity).# Belongs to the Bcl-2 family.
SIZE: 41 amino acids; 4678 Da
SUBUNIT: Interacts with HN.
SUBCELLULAR LOCATION: Cytoplasm.
DOMAIN: SwissProt: Q07815
DISEASE:SwissProt: Q07812 # Defects in BAX are found in some cell lines from hematopoietic malignancies as T-cell acute lymphoblastic leukemia, Burkitt lymphoma, and plasmacytoma.
SIMILARITY: SwissProt: Q07815 ## Belongs to the Bcl-2 family. | Q07814 # Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.# Belongs to the Bcl-2 family. | P55269 ## Belongs to the Bcl-2 family. | Q07812 # Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family (By similarity).# Belongs to the Bcl-2 family.
Brand Family:
Upstate
Product Name:
Anti-Bax, NT
Concentration:
1 mg/mL
Antibody Type:
Polyclonal Antibody
Qty/Pk:
200 µg
Format:
Purified
Host:
Rabbit