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WB: HL-60 cell lysate probed with anti-caspase1 at 1μg/ml.
Anti-Caspase 1
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R, M | IC, IP, WB | Rabbit | Purified | Polyclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: P29466 # Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.
SIZE: 404 amino acids; 45159 Da
SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p1 ... see more »
SIZE: 404 amino acids; 45159 Da
SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p1 ... see more »
Entrez Gene Number:
Entrez Gene Summary
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleu
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Description:
Anti-Caspase 1
Background Information:
Caspase-1, also known as IL-1 beta-converting enzyme (ICE), is an ezyme that cleaves the inflammatory cytokines IL-1 beta and IL-18, releasing the mature, active peptides. Caspase-1 is important for defense against pathogens and it also is involved in the induction of apoptosis. Thymocytes from caspase-1 deficient mice are resistant to Fas-mediated apoptosis, and caspase-1 is similar to the cell death gene CED-3 of C. elegans. Caspase-1 is a heterotetramer that consists of two anti-parallel heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The enzyme has a preferred cleavage site of Tyr-Val-Ala-Asp (YVAD). Caspase-1 is highly expressed in spleen and lung, and is absent from brain.
Alternate Names:
- CASP1 nirs variant 1
- IL-1 beta-converting enzyme
- Interleukin-1 beta convertase
- Interleukin-1 beta-converting enzyme
- caspase 1
- caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase)
-
caspase 1, apoptosis-related cysteine protease (interleukin 1, beta,
convertase) - interleukin 1-B converting enzyme
- interleukin 1-beta convertase
Specificity:
Recognizes the p45 proenzyme, Mr 45 kDa, and the p20 subunit, Mr 20 kDa, of reduced human Caspase 1 isoforms.
Key Applications:
- Immunocytochemistry
- Immunoprecipitation
- Western Blotting
Application Notes:
Immunoprecipitation:
5 μg of a previous lot of this antibody immunoprecipitated p45 proenzyme form of Caspase 1 from 500 μg of human HL-60 cell lysates.
Immunocytochemistry:
10 μg/mL of a previous lot of this antibody gave positive immunostaining of HL60 cells.
5 μg of a previous lot of this antibody immunoprecipitated p45 proenzyme form of Caspase 1 from 500 μg of human HL-60 cell lysates.
Immunocytochemistry:
10 μg/mL of a previous lot of this antibody gave positive immunostaining of HL60 cells.
Species Reactivity:
- Human
- Rat
- Mouse
Species Reactivity Note:
Human, Mouse, Rat. Reactivity with other species has not been confirmed.
Isotype:
IgG
Immunogen:
KLH-conjugated, synthetic peptide corresponding to amino acid residues 129-149 of human Caspase 1 (PEHKTSDSTFLVFMSHGIREG).
Epitope:
a.a. 129-149
Molecular Weight:
Proenzyme (45 kDa) and p20 subunit (20 kDa)
Control:
Human HL-60
Presentation:
Purified rabbit IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl containing 0.05% sodium azide. Frozen solution.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Gene Symbol:
- CASP1
- IL1BCE
- P45
- IL-1BC
- IL1BC
- p45
- IL1B-convertase
- CASP-1
- ICE
Quality Assurance:
Routinely evaluated by Western Blot on cell lysate from human HL-60.
Western Blot Analysis:
0.5-2 μg/mL of this lot of antibody detected the p45 proenzyme form of Caspase 1 in 20 μg of cell lysate from human HL-60. The immuno-reactivity can be inhibited by the immunizing peptide.
Western Blot Analysis:
0.5-2 μg/mL of this lot of antibody detected the p45 proenzyme form of Caspase 1 in 20 μg of cell lysate from human HL-60. The immuno-reactivity can be inhibited by the immunizing peptide.
Antibody Category:
- Apoptosis & Cancer
- Metabolism
Antibody Sub-Category:
- Caspases
- Enzymes & Biochemistry
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Upstate (Millipore)
Format Code:
Pur
Concentration:
1 mg/mL
Antibody Type:
Polyclonal Antibody
Format:
Purified
Host:
Rabbit
Purification Method:
Protein A Purified