Content Loading...
Content Loading...
Product Images
WB: HL-60 cell lysate probed with anti-caspase1 at 1μg/ml.
Anti-Caspase 1
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R, M | IC, IP, WB | Rabbit | Purified | Polyclonal Antibody |
Description:
Anti-Caspase 1
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes the p45 proenzyme, Mr 45 kDa, and the p20 subunit, Mr 20 kDa, of reduced human Caspase 1 isoforms.
Molecular Weight:
Proenzyme (45 kDa) and p20 subunit (20 kDa)
Epitope:
a.a. 129-149
Immunogen:
KLH-conjugated, synthetic peptide corresponding to amino acid residues 129-149 of human Caspase 1 (PEHKTSDSTFLVFMSHGIREG).
Isotype:
IgG
Background Information:
Caspase-1, also known as IL-1 beta-converting enzyme (ICE), is an ezyme that cleaves the inflammatory cytokines IL-1 beta and IL-18, releasing the mature, active peptides. Caspase-1 is important for defense against pathogens and it also is involved in the induction of apoptosis. Thymocytes from caspase-1 deficient mice are resistant to Fas-mediated apoptosis, and caspase-1 is similar to the cell death gene CED-3 of C. elegans. Caspase-1 is a heterotetramer that consists of two anti-parallel heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The enzyme has a preferred cleavage site of Tyr-Val-Ala-Asp (YVAD). Caspase-1 is highly expressed in spleen and lung, and is absent from brain.
Species Reactivity:
- Human
- Rat
- Mouse
Species Reactivity Note:
Human, Mouse, Rat. Reactivity with other species has not been confirmed.
Application Notes:
Immunoprecipitation:
5 μg of a previous lot of this antibody immunoprecipitated p45 proenzyme form of Caspase 1 from 500 μg of human HL-60 cell lysates.
Immunocytochemistry:
10 μg/mL of a previous lot of this antibody gave positive immunostaining of HL60 cells.
5 μg of a previous lot of this antibody immunoprecipitated p45 proenzyme form of Caspase 1 from 500 μg of human HL-60 cell lysates.
Immunocytochemistry:
10 μg/mL of a previous lot of this antibody gave positive immunostaining of HL60 cells.
Control:
Human HL-60
Quality Assurance:
Routinely evaluated by Western Blot on cell lysate from human HL-60.
Western Blot Analysis:
0.5-2 μg/mL of this lot of antibody detected the p45 proenzyme form of Caspase 1 in 20 μg of cell lysate from human HL-60. The immuno-reactivity can be inhibited by the immunizing peptide.
Western Blot Analysis:
0.5-2 μg/mL of this lot of antibody detected the p45 proenzyme form of Caspase 1 in 20 μg of cell lysate from human HL-60. The immuno-reactivity can be inhibited by the immunizing peptide.
Purification Method:
Protein A purfied
Presentation:
Purified rabbit IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl containing 0.05% sodium azide. Frozen solution.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- CASP1
- IL1BCE
- P45
- IL-1BC
- IL1BC
- p45
- IL1B-convertase
- CASP-1
- ICE
Alternate Names:
- CASP1 nirs variant 1
- IL-1 beta-converting enzyme
- Interleukin-1 beta convertase
- Interleukin-1 beta-converting enzyme
- caspase 1
- caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase)
-
caspase 1, apoptosis-related cysteine protease (interleukin 1, beta,
convertase) - interleukin 1-B converting enzyme
- interleukin 1-beta convertase
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Immunocytochemistry
- Immunoprecipitation
- Western Blotting
Entrez Gene Summary:
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing of this gene results in five transcript variants encoding distinct isoforms.
UniProt Summary:
FUNCTION: SwissProt: P29466 # Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.
SIZE: 404 amino acids; 45159 Da
SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The p20 subunit can also form a heterodimer with the epsilon isoform which then has an inhibitory effect. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and NALP2 and whose function would be the activation of proinflammatory caspases.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.
PTM: The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
SIMILARITY: SwissProt: P29466 ## Belongs to the peptidase C14 family. & Contains 1 CARD domain. ... hide » see more »
SIZE: 404 amino acids; 45159 Da
SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The p20 subunit can also form a heterodimer with the epsilon isoform which then has an inhibitory effect. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and NALP2 and whose function would be the activation of proinflammatory caspases.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.
PTM: The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
SIMILARITY: SwissProt: P29466 ## Belongs to the peptidase C14 family. & Contains 1 CARD domain. ... hide » see more »
Brand Family:
Upstate
Product Name:
Anti-Caspase 1
Concentration:
1 mg/mL
Antibody Type:
Polyclonal Antibody
Qty/Pk:
200 µg
Format:
Purified
Host:
Rabbit