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Anti-Bak, NT
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R, M | IC, IH, IP, WB | Rabbit | Purified | Polyclonal Antibody |
Description:
Anti-Bak, NT
Trade Name:
Upstate (Millipore)
Specificity:
Specific for Bak, MW ~28 kDa.
Molecular Weight:
28 kDa
Epitope:
N-terminus
Immunogen:
Synthetic peptide (SEEQVAQQDTEEVFRSC) corresponding to amino acid residues 23-38 of human Bak with a cysteine residue added to the C-terminus for conjugation to KLH.
Isotype:
IgG
Background Information:
Bak or Bcl2 homologous antagonist is a member of the Bcl2 family of proteins. The Bcl-2 related proteins interact with one another through the formation of homo and heterodimers. The susceptibility of cells to apoptotic stimuli is thought to be controlled by the relative ratios of the different Bcl2 family proteins. Bak has been demonstrated to accelerate the rate of apoptosis in growth factor deprived murine lymphoid, neuronal and fibroblastic cell lines. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. Bak also interacts with the tumor suppressor P53 after exposure to cell stress.
Species Reactivity:
- Human
- Rat
- Mouse
Species Reactivity Note:
Human, mouse and rat.
Application Notes:
Immunohistochemistry (Paraffin):
5 μg/mL of a previous lot detected Bak in paraffin-embedded rat kidney tissue.
Immunoprecipitation:
4 μg of a previous lot immunoprecipitated Bak from 500 μg mouse 3T3 cell lysate.
5 μg/mL of a previous lot detected Bak in paraffin-embedded rat kidney tissue.
Immunoprecipitation:
4 μg of a previous lot immunoprecipitated Bak from 500 μg mouse 3T3 cell lysate.
Control:
Included Positive Antigen Control: Catalog # 12-301, non-stimulated human A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Quality Assurance:
Evaluated by Western Blot on HEK293 lysates.
Western Blotting Analysis:
1:500 dilution of this antibody detected Bak on 10 µg of HEK293 lysates.
Western Blotting Analysis:
1:500 dilution of this antibody detected Bak on 10 µg of HEK293 lysates.
Purification Method:
Protein A purfied
Presentation:
Purified rabbit polyclonal in storage buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide. Frozen liquid.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- BAK1
- BAK
- MGC3887
- BCL2L7
- BAK-LIKE
- OTTHUMP00000039620
- CDN1
- MGC117255
Alternate Names:
- Apoptosis regulator BAK
- BCL2-antagonist/killer 1
- BCL2-like 7 protein
- Bcl-2 homologous antagonist/killer
- Bcl-2-like 7 protein
- pro-apoptotic protein BAK
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Immunocytochemistry
- Immunohistochemistry
- Immunoprecipitation
- Western Blotting
Entrez Gene Summary:
The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress.
UniProt Summary:
FUNCTION: SwissProt: Q16611 # In the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the a repressor Bcl-2 or its adenovirus homolog E1B 19k protein.
SIZE: 211 amino acids; 23409 Da
SUBUNIT: Forms heterodimers with Bcl-2, E1B 19k protein, and Bcl- X(L).
SUBCELLULAR LOCATION: Membrane; Single-pass membrane protein (Potential).
TISSUE SPECIFICITY: Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle.
DOMAIN:SwissProt: Q16611 Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
SIMILARITY: Belongs to the Bcl-2 family.
SIZE: 211 amino acids; 23409 Da
SUBUNIT: Forms heterodimers with Bcl-2, E1B 19k protein, and Bcl- X(L).
SUBCELLULAR LOCATION: Membrane; Single-pass membrane protein (Potential).
TISSUE SPECIFICITY: Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle.
DOMAIN:SwissProt: Q16611 Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
SIMILARITY: Belongs to the Bcl-2 family.
Brand Family:
Upstate
Product Name:
Anti-Bak, NT
Concentration:
1 mg/mL
Antibody Type:
Polyclonal Antibody
Qty/Pk:
200 μg
Format:
Purified
Host:
Rabbit