Anti-iNOS/NOS II, NT

P35228

FUNCTION: SwissProt: P35228 # Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions.
COFACTOR: Heme group (By similarity). & Binds 1 FAD (By similarity). & Binds 1 FMN (By similarity). & Metrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme (By similarity).
SIZE: 1153 amino acids; 131117 Da
SUBUNIT: Homodimer. Binds SLC9A3R1.
TISSUE SPECIFICITY: Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.
SIMILARITY: SwissProt: P35228 ## Belongs to the NOS family. & Contains 1 flavodoxin-like domain. ... nascondi » visualizza altro »

NM_010927

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17.

Anti-iNOS/NOS II, NT

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. Human iNOS is regulated by calcium/calmodulin (in contrast with mouse NOS2).

• Hepatocyte NOS 3
• Inducible NO synthase 3
• Inducible NOS 3
• NOS type II 3
• NOS, type II 2
• nitric oxide synthase 2, inducible
• nitric oxide synthase 2A
• nitric oxide synthase 2A (inducible, hepatocytes)
• nitric oxide synthase, macrophage 2

Recognizes N-terminus of iNOS, Mr 125 kDa. Does not cross-react with nNOS/NOS I.

• Immunohistochemistry
• Immunoprecipitation
• Western Blotting

Immunohistochemistry:
A previous lot of this antibody has been reported to detect iNOS/NOS II in 2% paraformaldehyde-fixed sheep lung (Sherman, T.S., 1999).

Immunoprecipitation:
4 μg of a previous lot immunoprecipitated iNOS/NOS II from 500 μg of L929B cells which had been stimulated with 10 ng/mL IFNg and 1 μg/mL LPS.

• Mouse
• Bovine
• Sheep
• Guinea Pig

Mouse, bovine, sheep and guinea pig. Other species cross-reactivity is unknown.

IgG

Partial fusion protein raised against the N-terminus of murine iNOS/NOS II.

N-terminus

125 kDa

INFγ and LPS treated L929B cell lysate, cytokine stimulated A549 cells, cytokine stimulated rat fibroblasts

Purified IgG in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, and 0.1% sodium azide. Frozen solution.

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

• EC 1.14.13.39
• HEP-NOS
• INOS
• NOS
• NOS2A
• iNOS

Routinely evaluated by immunoblot on lysates from L929B cells that had been stimulated IFNγ and LPS.

Western Blot Analysis: 1-4 μg/mL of this lot detected iNOS/NOS II in RIPA lysates from RAW 264.7 cells that had been stimulated with LPS. 2 μg/mL of a previous lot detected iNOS/NOS II from similarly treated L8 cells.

Neuroscience

Oxidative Stress

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Upstate (Millipore)

Pur

1 mg/mL

Polyclonal Antibody

Purified

Rabbit

Note: ADMA (Catalog #19-169) and NMMA (Catalog #19-170) are inhibitors of NOS

Protein A