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Catalogue Number : 06-583
Qty/Pk : 200 µg
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12-303	Jurkat Cell Lysate	100 µL

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WB: Jurkat cell lysate probed with anti-Lck at 1µg/ml

Anti-Lck

Species Reactivity	Key Applications	Host	Format	Antibody Type
B, H, M	IP, WB	Rabbit	Purified	Polyclonal Antibody

UniProt Number:

P06239

UniProt Summary

FUNCTION: SwissProt: P06239 # Tyrosine kinase that plays an essential role for the selection and maturation of developing T-cell in the thymus and in mature T-cell function. Is constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors and plays a key role in T-cell antigen receptor(TCR)-linked signal transduction pathways. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, and thereby recruits the associated LCK to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosines-based activation motifs (ITAMs) in the cytoplasmic tails of the TCRgamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. In addition, contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, and upon engagement of the CD2 molecule, LCK undergoes hyperphosphorylation and activation. Also plays a role in the IL2 receptor-linked signaling pathway that controls T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR.

SIZE: 509 amino acids; 58001 Da

SUBUNIT: Binds to the cytoplasmic domain of cell surface receptors, such as CD2, CD4, CD5, CD8, CD44, CD45 and CD122. Also binds to effector molecules, such as PI4K, VAV1, RASA1, FYB and to other protein kinases including CDC2, RAF1, ZAP70 and SYK. Binds to phosphatidylinositol 3'-kinase (PI3K) from T-lymphocytes through its SH3 domain and to the tyrosine phosphorylated form of KHDRBS1/p70 through its SH2 domain. Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine-kinase activity. Interacts with SQSTM1. Interacts with phosphorylated LIME1. Interacts with CBLB.

SUBCELLULAR LOCATION: Cytoplasm. Cell membrane; Lipid-anchor; Cytoplasmic side. Note=Present in lipid rafts in an unactive form.

TISSUE SPECIFICITY: Expressed specifically in lymphoid cells.

DOMAIN: SwissProt: P06239 The SH2 domain mediates interaction with SQSTM1. Interaction is regulated by Ser-59 phosphorylation.

PTM: Phosphorylated on Tyr-394, which increases enzymatic activity (By similarity). Phosphorylated on Tyr-505, which decreases activity.
DISEASE:SwissProt: P06239 # A chromosomal aberration involving LCK is found in leukemias. Translocation t(1;7)(p34;q34) with TCRB.

SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain. ... see more »

Entrez Gene Number:

NM_005356

Entrez Gene Summary

This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds ... see more »

Description:

Anti-Lck

Specificity:

Lck; reactivity with other Src family members not determined

Key Applications:

Immunoprecipitation
Western Blotting

Species Reactivity:

Bovine
Human
Mouse

Isotype:

IgG

Immunogen:

GST fusion protein corresponding to amino acids 1-58 of human Lck

Molecular Weight:

56kDa

Presentation:

0.1M Tris-glycine, pH 7.4, 0.15M NaCl with 0.05% sodium azide

Storage Conditions:

2 years at -20°C

Gene Symbol:

LCK
p56lck
YT16
pp58lck
p56-LCK
LSK

Quality Assurance:

routinely evaluated by immunoblot on RIPA lysates from Jurkat cells

Antibody Category:

Cell Signaling

Antibody Sub-Category:

Immunological Signaling

Usage Statement:

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Purification Method:

protein A chromatography

Format:

Purified

Antibody Type:

Polyclonal Antibody

Host:

Rabbit