Anti-β-Catenin
β-catenin plays a central role in cell adhesion, bridging between cadherins and actin cytoskeleton. These activities are regulated through its differential interaction with several molecular partners, including cell-cell adherens junction (AJ) molecules, components of its degradation complex, and transcription factors.
β-Catenin is composed of three domains: a central armadillo repeat domain and two N- and C-terminal tails. β-catenin can be phosphorylated by serine-threonine kinase, such as GSK-3. The phosphorylated β-catenin affects the protein association of β-catenin to E-cadherin and α-cateinin that occurs in the cell. This process can be reversed by de-phosphorylation of β-catenin.
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catenin (cadherin-associated protein), beta 1 (88kD)
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catenin (cadherin-associated protein), beta 1 (88kDa)
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catenin (cadherin-associated protein), beta 1, 88kDa
Recognizes β-Catenin, Mr 92 kDa.
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Immunocytochemistry
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Immunoprecipitation
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Western Blotting
Immunoprecipitation:
5 µg of a previous lot immunoprecipitated β-Catenin from 500 µg of A431 membrane fraction lysate.
Immunocytochemistry:
1-5 µg/mL of a previous lot showed positive immunostaining for β-catenin at cell-cell junctions of A431 cells fixed with 4% paraformaldehyde and permeabilized with 0.25% Triton® X-100.
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Human
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Rat
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Mouse
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Rabbit
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Bovine
Human, bovine, rat, rabbit and mouse.
IgG
KLH-conjugated, synthetic peptide (CGG-SYLDSGIHSGATTTAPSLSGK) corresponding to the consensus GSK3 phosphorylation site of human β -Catenin (amino acids 29-49).
92 kDa
A431 cell lysate
Included Positive Antigen Control: Catalog #12-301, Non stimulated A431 cell lysate. Add 2.5 µL of 2-mercaptoethanol/100 µL of lysate and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced lysate per lane for minigels.
Purified rabbit polyclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide and 30% glycerol. Store at -20°C.
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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CTNNB1
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CTNNB
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Beta-catenin
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PRO2286
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FLJ37923
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FLJ25606
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DKFZp686D02253
Routinely evaluated by western blot on A431 total cell lysate.
Western Blot Analysis:
0.1-1 µg/mL of this lot detected β-catenin in an A431 total cell lysate.
Nuclear Function
Transcription Factors
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Upstate (Millipore)
Pur
1 mg/mL
Polyclonal Antibody
Purified
Rabbit
Protein A chromatography
