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WB: 3T3/NIH cell lysate was probed with anti-phospho-Adducin (Ser662) (2μg/ml).
Anti-phospho-Adducin (Ser662)
| 交差性 | 主要アプリケーション | 免疫動物 | フォーマット | 抗体タイプ |
|---|---|---|---|---|
| Ca, H, M, R | WB | Rabbit | Affinity Purified | Polyclonal Antibody |
Description:
Anti-phospho-Adducin (Ser662)
Trade Name:
Upstate (Millipore)
Specificity:
phosphorylated serine 662 of γ-Adducin, phosphorylated serine 724 of α-Adducin, phosphorylated serine 713 of β-Adducin
Molecular Weight:
80kDa, 120kDa, 110kDa
Immunogen:
peptide (C-KKFRTPpSFLKKNK) corresponding to amino acids 656-668 of human gamma-Adducin
Modifications:
Phosphorylation
Isotype:
IgG
Species Reactivity:
- Canine
- Human
- Mouse
- Rat
Quality Assurance:
routinely evaluated by immunoblot on RIPA lysates from 3T3/NIH cells or on canine MDCK cells
Purification Method:
Immunoaffinity purified
Presentation:
0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide, and 30% glycerol. Liquid at -20°C.
Storage Conditions:
1 year at -20°C
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- ADD3
- adducin-like
- ADDL
- Gamma-adducin
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced adducin gamma transcripts encoding different isoforms have been described. The functions of the different isoforms are not known.
Key Applications:
Western Blotting
UniProt Summary:
FUNCTION: SwissProt: Q9UEY8 # Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
SIZE: 706 amino acids; 79155 Da
SUBUNIT: Heterodimer of an alpha and a gamma subunit.
TISSUE SPECIFICITY: Heart only expresses isoform 1.
DOMAIN:SwissProt: Q9UEY8 Comprised of three regions: a N-terminal protease- resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.
PTM: Sumoylated.
SIMILARITY: Belongs to the aldolase class II family. Adducin subfamily.
SIZE: 706 amino acids; 79155 Da
SUBUNIT: Heterodimer of an alpha and a gamma subunit.
TISSUE SPECIFICITY: Heart only expresses isoform 1.
DOMAIN:SwissProt: Q9UEY8 Comprised of three regions: a N-terminal protease- resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.
PTM: Sumoylated.
SIMILARITY: Belongs to the aldolase class II family. Adducin subfamily.
Qty/Pk:
100 µg
Brand Family:
Upstate
Format:
Affinity Purified
Host:
Rabbit
Product Name:
Anti-phospho-Adducin (Ser662)
Antibody Type:
Polyclonal Antibody