Product Images
WB: 20 micrograms of Hela nuclear extract was probed with 0.5μg/ml of anti-CREB.
Anti-CREB Antibody
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | IP, WB | Rabbit | Purified | Polyclonal Antibody |
Description:
Anti-CREB Antibody | 06-863
Promotional Text:
Trade Name:
Upstate (Millipore)
Specificity:
This antibody recognizes CREB.
Molecular Weight:
43 kDa
Epitope:
a.a. 5-24
Immunogen:
KLH-conjugated, synthetic peptide (CSGAENQ QSGDAAVTEAENQQ) corresponding to a.a. 5-24 of human CREB. The immunizing sequence is identical in pig, shares 19 of 20 residues with mouse and bovine and 18 of 20 residues with rat.
Isotype:
IgG
Background Information:
CREB is a β-ZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations. CREB promotes outgrowth and differentiation as a mediator of Neurotrophin pathways. Additionally, CREB signaling is involved in learning and memory in several organisms. CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV and MAPKAPK-2.
Species Reactivity:
- Human
- Mouse
Species Reactivity Note:
Human and mouse.
Broad species cross-reactivity expected due to conservation of immunogenic sequence.
Broad species cross-reactivity expected due to conservation of immunogenic sequence.
Application Notes:
Immunoprecipitation:
5 μg of this lot immunoprecipitated CREB from 500 μg of HeLa nuclear extract.
5 μg of this lot immunoprecipitated CREB from 500 μg of HeLa nuclear extract.
Control:
HeLa nuclear extract
Included Positive Antigen Control: Catalog # 12-309, HeLa nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 5-10 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Included Positive Antigen Control: Catalog # 12-309, HeLa nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 5-10 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Quality Assurance:
Routinely evaluated by western blot on HeLa nuclear lysate.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected CREB from a HeLa nuclear lysate.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected CREB from a HeLa nuclear lysate.
Purification Method:
Protein A purfied
Presentation:
Purified rabbit IgG in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% sodium azide, and 30% glycerol.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing cap.
For maximum recovery of product, centrifuge the vial prior to removing cap.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- MGC9284
- CREB1
Alternate Names:
- active transcription factor CREB
- cAMP responsive element binding protein 1
- cAMP-response element-binding protein-1
- transactivator protein
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Immunoprecipitation
- Western Blotting
Entrez Gene Summary:
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms.
UniProt Summary:
FUNCTION: SwissProt: P16220 # This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE.
SIZE: 341 amino acids; 36688 Da
SUBUNIT: Binds DNA as a dimer. Interacts PPRC1 and with HTLV-1 Tax-1.
SUBCELLULAR LOCATION: Nucleus.
PTM: Stimulated by phosphorylation. Phosphorylation of both Ser- 133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP and EP300 binding (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: SwissProt: P16220 ## Belongs to the bZIP family. & Contains 1 bZIP domain. & Contains 1 KID (kinase-inducible) domain.
SIZE: 341 amino acids; 36688 Da
SUBUNIT: Binds DNA as a dimer. Interacts PPRC1 and with HTLV-1 Tax-1.
SUBCELLULAR LOCATION: Nucleus.
PTM: Stimulated by phosphorylation. Phosphorylation of both Ser- 133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP and EP300 binding (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: SwissProt: P16220 ## Belongs to the bZIP family. & Contains 1 bZIP domain. & Contains 1 KID (kinase-inducible) domain.
Brand Family:
Upstate
Product Name:
Anti-CREB Antibody | 06-863
Concentration:
1 mg/mL
Antibody Type:
Polyclonal Antibody
Qty/Pk:
200 µg
Format:
Purified
Host:
Rabbit