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Ordering Information

  • : 06-866
  • : 200 µL
  • : Please refer to the Certificate of Analysis for the lot-specific concentration.
  • Product Family Information

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WB: 10 micrograms of acid extracts of untreated (lane 1) or butyrate-treated (lane 2) HeLa cells probed with Anti-acetyl Histone H4 at a 1:2000 dilut...

Immunocytochemistry:
Representative lot data.
Confocal fluorescent analysis of HeLa and NIH/3T3 cells using 06-866 (Red). Actin filaments h...

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Anti-acetyl-Histone H4 Antibody

Species Reactivity Key Applications Host Format Antibody Type
H, T, Eu WB, ICC, ChIP Rabbit Serum Polyclonal Antibody
Description:
Anti-acetyl-Histone H4 Antibody
Promotional Text:
Trade Name:
Upstate (Millipore)
Specificity:
Acetylated histone H4, acetylated histone H2B from Tetrahymena and weakly cross-reacts with acetylated histone H2B from HeLa cells, may cross-react with other acetylated proteins.
Molecular Weight:
10 kDa
Epitope:
a.a. 2-19
Immunogen:
KLH-conjugated peptide corresponding to a.a. 2-19 of Tetrahymena histone H4 [AGGAcKGGAcKGMGAcKVGAAcKRHS-C], acetylated on lysines 5, 8, 12 and 16.
Modifications:
Acetylation
Isotype:
Background Information:
Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
Species Reactivity:
  • Human
  • Tetrahymena
  • Eukaryote
Species Reactivity Note:
Human and Tetrahymena. Other species not tested, but expected to cross-react since Histone H4 is well conserved.
Application Notes:
Chromatin Immunoprecipitation:
5-10 μL of this lot immunoprecipitated transcriptionally active chromatin containing acetylated histone H4 from 2 X 106 serum stimulated HeLa cells.
Control:
TSA-treated Jurkat cells. For a negative control, perform a no-antibody immunoprecipitation by incubating the supernatant fraction with 60μl of Salmon Sperm DNA/Protein A Agarose- 50% Slurry. Transcriptionally unactivated DNA samples should be prepared as controls for PCR.
Quality Assurance:
Routinely evaluated by western blot on Tetrahymena macronuclei or acid extracted proteins from HeLa cells treated with 5 mM sodium butyrate.

Western Blot Analysis:
1:2000 dilution of this lot detected acetylated histone H4 in acid extracted proteins from HeLa cells treated with 5 mM sodium butyrate. Sodium butyrate, an inhibitor of deacetylases, was used to enhance detection of acetylated histone H4.
Purification Method:
Unpurified
Presentation:
Whole antiserum containing 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
  • HIST1H4A
  • H4/J
  • HIST1H4F
  • HIST1H4L
  • H4FN
  • H4FA
  • H4FH
  • HIST1H4H
  • H4F2
  • H4FM
  • H4/A
  • H4FK
  • H4FG
  • HIST2H4
  • H4FB
  • H4/K
  • HIST1H4I
  • H4/N
  • HIST1H4B
  • H4FE
  • H4/M
  • H4/a
  • IST1H4E
  • HIST4H4
  • HIST2H4A
  • H4FC
  • H4FI
  • H4/H
  • HIST1H4C
  • H4/G
  • HIST1H4K
  • 4FJ
  • H4FD
  • H4/I
  • H4/B
  • H4/D
  • H4/C
  • HIST1H4J
  • HIST1H4D
  • H4/E
Alternate Names:
  • H4 histone family, member A
  • histone 1, H4a
  • histone cluster 1, H4a
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
  • Western Blotting
  • Immunocytochemistry
  • Chromatin Immunoprecipitation (ChIP)
Entrez Gene Summary:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
UniProt Summary:
FUNCTION: SwissProt: P62805 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
SIZE: 103 amino acids; 11367 Da
SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
SUBCELLULAR LOCATION: Nucleus.
PTM: Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage (By similarity). & Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
SIMILARITY: SwissProt: P62805 ## Belongs to the histone H4 family.
Brand Family:
Upstate
Product Name:
Anti-acetyl-Histone H4 Antibody
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Polyclonal Antibody
Qty/Pk:
200 µL
Format:
Serum
Host:
Rabbit
Applications:
Use Anti-acetyl-Histone H4 Antibody (Rabbit Polyclonal Antibody) validated in ChIP, WB, ICC to detect acetyl-Histone H4 also known as H4 histone family member A, histone 1-H4a.

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