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WB: rat brain microsomal fraction was incubated without (lane 1) and with lambda phosphatase (lane 2) before being probed with Anti-phospho-CaM Kinase...
Anti-phospho-CaM Kinase II, α/β subunit (Thr286/287)
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R | WB | Rabbit | Purified | Polyclonal Antibody |
Description:
Anti-phospho-CaM Kinase II, α/β subunit (Thr286/287)
Trade Name:
Upstate (Millipore)
Specificity:
phospho-CaM Kinase II alpha and beta subunits
Molecular Weight:
50kDa (alpha) and 60kDa (beta)
Immunogen:
peptide (K-MHRQE[pT]VDCLK) corresponding to amino acids 281-291 of rat calmodulin-dependent protein kinase II, α subunit
Modifications:
Phosphorylation
Isotype:
IgG
Species Reactivity:
- Mouse
- Rat
Quality Assurance:
routinely evaluated by immunoblot on a rat brain microsomal preparation either treated or non-treated with lambda phosphatase
Purification Method:
Protein A chromatography
Presentation:
0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
Storage Conditions:
2 years at -20°C
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- CAMK2A
- KIAA0968
- CAMKA
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
The product of this gene belongs to the Serine/Threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This enzyme is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for this gene.
Key Applications:
Western Blotting
UniProt Summary:
FUNCTION: SwissProt: Q9UQM7 # CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity (By similarity).
SIZE: 478 amino acids; 54030 Da
SUBUNIT: CAMK2 is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. Interacts with BAALC, MPDZ, SYN1 and SYNGAP1 (By similarity).
SUBCELLULAR LOCATION: Cell junction, synapse, presynaptic cell membrane (By similarity). Cell junction, synapse (By similarity). Note=Postsynaptic lipid rafts (By similarity).
SIMILARITY: SwissProt: Q9UQM7 ## Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. & Contains 1 protein kinase domain.
SIZE: 478 amino acids; 54030 Da
SUBUNIT: CAMK2 is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. Interacts with BAALC, MPDZ, SYN1 and SYNGAP1 (By similarity).
SUBCELLULAR LOCATION: Cell junction, synapse, presynaptic cell membrane (By similarity). Cell junction, synapse (By similarity). Note=Postsynaptic lipid rafts (By similarity).
SIMILARITY: SwissProt: Q9UQM7 ## Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. & Contains 1 protein kinase domain.
Qty/Pk:
200 µg
Brand Family:
Upstate
Format:
Purified
Host:
Rabbit
Product Name:
Anti-phospho-CaM Kinase II, α/β subunit (Thr286/287)
Antibody Type:
Polyclonal Antibody