Anti-NFκB p50 Antibody

Anti-NFκB p50 Antibody | 06-886

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Upstate (Millipore)

Recognizes NFκB p50, MW 50 kDa and its 105 kDa precursor.

Varies

KLH conjugated synthetic peptide (MAEDDPYLGRPE-C) corresponding to amino acids 1-12 of NFκB p50, containing a C-terminal cysteine.

IgG

Proteins encoded by the v-Rel viral oncogene and its cellular homolog, c-Rel, are members of a family of transcription factors that include the two subunits of the transcription factor NFκB (p50 and p65) and the Drosophila maternal morphogen, dorsal. Both proteins specifically bind to DNA sequences that are the same or slight variations of the 10 bp κB sequence in the immunoglobulin κ light chain enhancer. This same sequence is also present in a number of other cellular and viral enhancers. The DNA binding activity of NFκB is activated and NFκB is subsequently transported from the cytoplasm to the nucleus in cells exposed to mitogens or growth factors. cDNAs encoding precursors for two distinct proteins of the same size have been described, designated p105 and p100. The p105 precursor contains p50 at its N-terminus and a C-terminal region that when expressed as a separate molecule, designated pdI, binds to p50 and regulates its activity.

• Human
• Mouse

Human, weak in mouse, none in rat.

Immunoprecipitation: 5 μL of a previous lot immunoprecipitated NFκB p50 from 500 μg of EGF-stimulated A431 RIPA lysate.

A431 cell lysate
Included Positive Antigen Control: Catalog # 12-302. EGF-stimulated A431 lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

Evaluated by western blot on a RIPA lysate from EGF-stimulated A431 cells.

Western Blot Analysis: 1:5000-1:10,000 dilution of this antibody detected NFκB p50 in a RIPA lysate from EGFstimulated A431 cells.

Unpurified

Whole rabbit antiserum containing 0.05% sodium azide and 30% glycerol.
Liquid at -20°C.

Stable for 1 year at from date of receipt.

P19838

NP_003989

• NFKB1
• NFKB-p105
• DKFZp686C01211
• NF-kappa-B
• EBP-1
• KBF1
• MGC54151
• NFKB-p50

• DNA binding factor KBF1
• DNA-binding factor KBF1
• nuclear factor NF-kappa-B p50 subunit
• nuclear factor kappa-B DNA binding subunit
• nuclear factor kappa-B, subunit 1
• nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

• Western Blotting
• Immunoprecipitation
• Chromatin Immunoprecipitation (ChIP)

This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth.

FUNCTION: SwissProt: P19838 # Appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins and generation of p50 by a cotranslational processing. The proteasome-mediated process ensures the production of both p50 and p105 and preserves their independent function, although processing of NFKB1/p105 also appears to occur post-translationally. p50 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. Plays a role in the regulation of apoptosis.
SIZE: 968 amino acids; 105356 Da
SUBUNIT: Active NF-kappa-B is a heterodimer of an about 50 kDa DNA-binding subunit and the weak DNA-binding subunit p65. Two heterodimers might form a labile tetramer. Also interacts with MAP3K8. NF-kappa-B p50 subunit interacts with NCOA3 coactivator, which may coactivate NF-kappa-B dependent expression via its histone acetyltransferase activity. Interacts with DSIPI; this interaction prevents nuclear translocation and DNA-binding. Interacts with SPAG9 and UNC5CL.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B).
DOMAIN: SwissProt: P19838 The C-terminus of p105 might be involved in cytoplasmic retention, inhibition of DNA-binding, and transcription activation. & Glycine-rich region (GRR) appears to be a critical element in the generation of p50.
PTM: While translation occurs, the particular unfolded structure after the GRR repeat promotes the generation of p50 making it an acceptable substrate for the proteasome. This process is known as cotranslational processing. The processed form is active and the unprocessed form acts as an inhibitor (I kappa B-like), being able to form cytosolic complexes with NF-kappa B, trapping it in the cytoplasm. Complete folding of the region downstream of the GRR repeat precludes processing. & Polyubiquitination seems to allow p105 processing.
SIMILARITY: Contains 7 ANK repeats. & Contains 1 death domain. & Contains 1 RHD (Rel-like) domain.

Upstate

Anti-NFκB p50 Antibody | 06-886

Variable

Polyclonal Antibody

150 µL

Serum

Rabbit