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Ordering Information

  • : 07-001
  • : 200 μL
  • : Please refer to the Certificate of Analysis for the lot-specific concentration.

Product Images

WB: HL-60 cell lysate was probed with anti-p47-phox (1:2000 dilution). Arrow indicates p47-phox.

Anti-p47-phox Antibody

Species Reactivity Key Applications Host Format Antibody Type
H ICC, IP, WB Rabbit Serum Polyclonal Antibody
Description:
Anti-p47-phox Antibody
Promotional Text:
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes p47 phox.
Molecular Weight:
47 kDa
Immunogen:
GST fusion protein corresponding to full-length human p47-phox
Isotype:
Species Reactivity:
Human
Control:
HL-60 cell lysate
Quality Assurance:
routinely evaluated by immunoblot on RIPA lysates from HL-60 cells
Purification Method:
Unpurified
Presentation:
Immunoaffinity Purified immunoglobulin in 0.2M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide, 5mg/ml BSA before the addition of glycerol to 30%
Storage Conditions:
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
  • NCF1
  • NCF-47K
  • NOXO2
  • p47-phox
  • SH3PXD1A
  • NCF1A
  • p47phox
  • NCF-1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
  • Immunocytochemistry
  • Immunoprecipitation
  • Western Blotting
Entrez Gene Summary:
The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease.
UniProt Summary:
FUNCTION: SwissProt: P14598 # NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
SIZE: 390 amino acids; 44683 Da
SUBCELLULAR LOCATION: Cytoplasm.
DISEASE:SwissProt: P14598 # Defects in NCF1 are the cause of autosomal cytochrome-b- positive chronic granulomatous disease type 1 (CGD) [MIM:233700]; an autosomal recessive form.
SIMILARITY: SwissProt: P14598 ## Contains 1 PX (phox homology) domain. & Contains 2 SH3 domains.
Brand Family:
Upstate
Product Name:
Anti-p47-phox Antibody
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Polyclonal Antibody
Qty/Pk:
200 μL
Format:
Serum
Host:
Rabbit
Applications:
Anti-p47-phox Antibody detects level of p47-phox & has been published & validated for use in IC, IP & WB.

Product Resources

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