Anti-IKKα Antibody (07-1007)

Anti-IKKα Antibody | 07-1007

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This antibody recognizes IKKα.

~80-85 kDa

KLH-conjugated linear peptide corresponding to IKKα.

The TNF and IL-1 signaling pathways activate the IκB kinases, IKKα (IKK1), IKKβ (IKK2). Phosphorylation of the IκB cytoplasmic inhibitors, IκBα, IκBβ and IκBγ by these kinases triggers proteolytic degradation and the release of NFκB/Rel proteins from the IκB inhibitors. This allows the NFκB proteins to enter the nucleus. A third member of the family, IKKε (IKKI), is not induced by TNF or IL-1. IKKε together with TANK-binding kinase-1 (TBK1), is essential for resistance to viral infection; either kinase can phosphorylate IRF3 and IRF7, leading to their nuclear translocation and induction of interferon and ISG56 gene expression.

• Human
• Canine
• Horse
• Bovine
• Rat
• Mouse

Proven to react with human. Predicted to react with rat, mouse, equine (horse), canine (dog), and bovine (cow) based on sequence homology.

Immunoprecipitation Analysis: 5-20 µg from a previous lot was used in IP.
Western Blot Analysis (SNAP ID Technique): 1 µg/mL from a previous lot detected IKKα in 10 µg of MCF-7 cell lysate.

MCF-7 cell lysate

Evaluated by Western Blot in MCF-7 cell lysate.
Western Blot Analysis: 1-4 µg/mL of this antibody detected IKKα in 10 µg of MCF-7 cell lysate.

Affinity Purfied

Purified rabbit polyclonal in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.150 mM NaCl and 0.05% sodium azide.

Stable at 2-8°C for 1 year from date of receipt.

O15111

NP_001269

• EC 2.7.11.10
• IKBKA
• IKK-A
• IKK-alpha
• IKK1
• IKKA
• IkBKA
• NFKBIKA
• OTTHUMP00000020273
• TCF16

• I kappa-B kinase alpha
• I-kappa-B kinase 1
• I-kappa-B kinase-alpha
• IKK-a kinase
• IkB kinase alpha subunit
• IkappaB kinase
• Nuclear factor NF-kappa-B inhibitor kinase alpha
• Nuclear factor NFkappaB inhibitor kinase alpha
• conserved helix-loop ubiquitous kinase
• conserved helix-loop-helix ubiquitous kinase

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

• Western Blotting
• Immunoprecipitation

This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor.

FUNCTION: Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines.
CATALYTIC ACTIVITY: ATP + [I-kappa-B protein] = ADP + [I-kappa-B phosphoprotein].
ENZYME REGULATIION: Activated when phosphorylated and inactivated when dephosphorylated.
SUBUNIT STRUCTURE: Component of the I-kappa-B-kinase (IKK) core complex consisting of CHUK, IKBKB and IKBKG; probably four alpha/CHUK-beta/IKBKB dimers are associated with four gamma/IKBKG subunits. The IKK core complex seems to associate with regulatory or adapter proteins to form a IKK-signalosome holo-complex. Part of a complex composed of NCOA2, NCOA3, CHUK/IKKA, IKBKB, IKBKG and CREBBP. Part of a 70-90 kDa complex at least consisting of CHUK/IKKA, IKBKB, NFKBIA, RELA, IKBKAP and MAP3K14. Directly interacts with IKK-gamma/NEMO and TRPC4AP By similarity. May interact with TRAF2. Interacts with NALP2. May interact with MAVS/IPS1.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note: Shuttles between the cytoplasm and the nucleus.
TISSUE SPECIFICITY: Widely expressed.
PTM: Phosphorylated by MAP3K14/NIK, AKT and to a lesser extent by MEKK1, and dephosphorylated by PP2A. Autophosphorylated.
SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
Contains 1 protein kinase domain.

Anti-IKKα Antibody (07-1007)

0.5 mg/mL

Polyclonal Antibody

150 µg

Purified

Rabbit