Ordering Information
- : 07-104
- : 100 μg
- : 1.0 mg/mL
Product Images
Western Blot Analysis:
Representative lot data.
HEK293 cell lysate was resolved by electrophoresis, transferred to PVDF membranes and probe...
Western Blot (Snap ID) Analysis:
Representative lot data.
HEK293 cell lysate was resolved by electrophoresis, transferred to PVDF membran...
Anti-IGF2 mRNA-binding protein 3
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, Chp, Ca, Eq | WB | Rabbit | Affinity Purified | Polyclonal Antibody |
Description:
Anti-IGF2 mRNA-binding protein 3 Antibody | 07-104
Promotional Text:
Specificity:
This antibody recognizes IGF2 mRNA-binding protein 3 at the C-terminus.
Molecular Weight:
64 kDa was observed. Predicted to cross-react with all the Isoforms of IGF2BP3.
Epitope:
C-terminus
Immunogen:
KLH-conjugated linear peptide corresponding to human IGF2 mRNA-binding protein 3 at and around the C-terminus.
Background Information:
The protein encoded by this gene is primarily found in the nucleolus, where it can bind to the 5' UTR of the insulin-like growth factor 2 leader 3 mRNA and may repress translation of insulin-like growth factor 2 during late development. The encoded protein contains several KH domains, which are important in RNA binding and are known to be involved in RNA synthesis and metabolism. A pseudogene exists on chromosome 7, and there are putative pseudogenes on other chromosomes. [provided by RefSeq].
Species Reactivity:
- Human
- Mouse
- Chimpanzee
- Canine
- Horse
Species Reactivity Note:
Reacts with human and mouse. Predicted to react with chimpanzee, Rhesus monkey, dog, and horse based on 100% sequence homology.
Application Notes:
Western Blot (SNAP ID) Analysis: 2 µg/mL from a previous lot detected IGF2 mRNA-binding protein 3 on 10 µg of Hek293 cell lysate.
Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP.
Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP.
Control:
Hek293 cell lysate
Quality Assurance:
Evaluated by Western Blot in Hek293 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected IGF2 mRNA-binding protein 3 on 10 µg of Hek293 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected IGF2 mRNA-binding protein 3 on 10 µg of Hek293 cell lysate.
Purification Method:
Affinity Purfied
Presentation:
Purified rabbit serum in PBS with 0.09% azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- IMP-3
- IMP3
- KOC1
- VICKZ3
- hKOC
- IGF2BP3
Alternate Names:
- IGF II mRNA binding protein 3
- IGF-II mRNA-binding protein 3
- IGF2 mRNA-binding protein 3
- KH domain containing protein overexpressed in cancer
- KH domain-containing protein overexpressed in cancer
- VICKZ family member 3
- insulin-like growth factor 2 mRNA binding protein 3
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Western Blotting
Entrez Gene Summary:
The protein encoded by this gene is primarily found in the nucleolus, where it can bind to the 5' UTR of the insulin-like growth factor 2 leader 3 mRNA and may repress translation of insulin-like growth factor 2 during late development. The encoded protein contains several KH domains, which are important in RNA binding and are known to be involved in RNA synthesis and metabolism. A pseudogene exists on chromosome 7, and there are putative pseudogenes on other chromosomes. [provided by RefSeq].
UniProt Summary:
FUNCTION: RNA-binding protein that act as a regulator of mRNA translation and stability. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binds to sequences in the 3'-UTR of CD44 mRNA.
SUBUNIT STRUCTURE: Homodimer and multimer.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note: Found in lamellipodia of the leading edge, in the perinuclear region, and beneath the plasma membrane. The subcytoplasmic localization is cell specific and regulated by cell contact and growth. Localized at the connecting piece and the tail of the spermatozoa. Colocalized with CD44 mRNA in RNP granules.
TISSUE SPECIFICITY: Expressed in fetal liver, fetal lung, fetal kidney, fetal thymus, fetal placenta, fetal follicles of ovary and gonocytes of testis, growing oocytes, spermatogonia and semen (at protein level). Expressed in cervix adenocarcinoma, in testicular, pancreatic and renal-cell carcinomas (at protein level). Expressed ubiquitously during fetal development at 8 and 14 weeks of gestation. Expressed in ovary, testis, brain, placenta, pancreatic cancer tissues and pancreatic cancer cell lines.
INVOLVEMENT IN DISEASE: Autoantibodies against IGF2BP3 are detected in sera from some patients with a variety of carcinomas.
SEQUENCE SIMILARITIES: Belongs to the RRM IMP/VICKZ family.
Contains 4 KH domains.
Contains 2 RRM (RNA recognition motif) domains.
SUBUNIT STRUCTURE: Homodimer and multimer.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note: Found in lamellipodia of the leading edge, in the perinuclear region, and beneath the plasma membrane. The subcytoplasmic localization is cell specific and regulated by cell contact and growth. Localized at the connecting piece and the tail of the spermatozoa. Colocalized with CD44 mRNA in RNP granules.
TISSUE SPECIFICITY: Expressed in fetal liver, fetal lung, fetal kidney, fetal thymus, fetal placenta, fetal follicles of ovary and gonocytes of testis, growing oocytes, spermatogonia and semen (at protein level). Expressed in cervix adenocarcinoma, in testicular, pancreatic and renal-cell carcinomas (at protein level). Expressed ubiquitously during fetal development at 8 and 14 weeks of gestation. Expressed in ovary, testis, brain, placenta, pancreatic cancer tissues and pancreatic cancer cell lines.
INVOLVEMENT IN DISEASE: Autoantibodies against IGF2BP3 are detected in sera from some patients with a variety of carcinomas.
SEQUENCE SIMILARITIES: Belongs to the RRM IMP/VICKZ family.
Contains 4 KH domains.
Contains 2 RRM (RNA recognition motif) domains.
Product Name:
Anti-IGF2 mRNA-binding protein 3
Concentration:
1.0 mg/mL
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 μg
Format:
Affinity Purified
Host:
Rabbit