Ordering Information
- : 07-1334
- : 100 µg
- : 1 mg/mL
Product Images
Immunocytochemistry Analysis: Confocal fluorescent analysis of TPA-treated HeLa cells using anti-JunD (Red). Actin filaments have been labeled with A...
Western Blot Analysis:
Representative lot data.
TPA-treated HeLa lysate was resolved by electrophoresis, transferred to PVDF and probed with...
Anti-JunD Antibody
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R, B | ICC, WB | Rabbit | Affinity Purified | Polyclonal Antibody |
Description:
Anti-JunD Antibody | 07-1334
Promotional Text:
Specificity:
This antibody recognizes JunD.
Molecular Weight:
~37 kDa
Immunogen:
KLH-conjugated linear peptide corresponding to human JunD.
Background Information:
JunD is a member of the JUN family, and a functional component of the AP1 transcription factor complex. JunD binds an AP-1 site and upon cotransfection stimulates the activity of a promoter that bears an AP-1 site. It also binds DNA as a dimer. Furthermore, JunD has been proposed to protect cells from p53-dependent senescence and apoptosis.
Species Reactivity:
- Human
- Mouse
- Rat
- Bovine
Species Reactivity Note:
Proven to react with human.
Predicted to react with mouse, rat, and cow based on 100% sequence homology.
Predicted to react with mouse, rat, and cow based on 100% sequence homology.
Application Notes:
Western Blot (SNAP ID Technique):
A 1:2,000 dilution from a previous lot detected JunD using the SNAP ID analysis.
Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected JunB in TPA-treated HeLa lysates.
Immunocytochemistry Analysis:
Representative images from a previous lot.
A 1:2,000 dilution from a previous lot detected JunD using the SNAP ID analysis.
Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected JunB in TPA-treated HeLa lysates.
Immunocytochemistry Analysis:
Representative images from a previous lot.
Control:
HeLa cell lysate
Quality Assurance:
Evaluated by Western Blot in TPA-treated HeLa cell lysate.
Western Blot Analysis: 0.25 µg/mL of this antibody detected JunD in 10 µg of TPA-treated HeLa cell lysate. Antibody will also work in untreated HeLa cells and lysate.
Western Blot Analysis: 0.25 µg/mL of this antibody detected JunD in 10 µg of TPA-treated HeLa cell lysate. Antibody will also work in untreated HeLa cells and lysate.
Purification Method:
Affinity Purfied
Presentation:
Purified rabbit polyclonal containing 0.1 M Tris-glycine, pH 7.4, 0.150 mM NaCl and 0.05% sodium azide.
Storage Conditions:
Stable at 2-8°C for 1 year from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
AP-1
Alternate Names:
- JunD-FL isoform
- activator protein 1
- jun D proto-oncogene
- transcription factor jun-D
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Immunocytochemistry
- Western Blotting
Entrez Gene Summary:
The protein encoded by this intronless gene is a member of the JUN family, and a functional component of the AP1 transcription factor complex. It has been proposed to protect cells from p53-dependent senescence and apoptosis. Alternate translation initiation site usage results in the production of different isoforms.
UniProt Summary:
FUNCTION: Binds an AP-1 site and upon cotransfection stimulates the activity of a promoter that bears an AP-1 site.
SUBUNIT STRUCTURE: Binds DNA as a dimer By similarity.
SUBCELLULAR LOCATION: Nucleus.
SEQUENCE SIMILARITIES: Belongs to the bZIP family. Jun subfamily.
Contains 1 bZIP domain.
SEQUENCE CAUTION: The sequence CAA40010.1 differs from that shown. Reason: Frameshift at several positions.
SUBUNIT STRUCTURE: Binds DNA as a dimer By similarity.
SUBCELLULAR LOCATION: Nucleus.
SEQUENCE SIMILARITIES: Belongs to the bZIP family. Jun subfamily.
Contains 1 bZIP domain.
SEQUENCE CAUTION: The sequence CAA40010.1 differs from that shown. Reason: Frameshift at several positions.
Product Name:
Anti-JunD Antibody (07-1334)
Concentration:
1 mg/mL
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 µg
Format:
Affinity Purified
Host:
Rabbit