Ordering Information
- : 07-1348
- : 100 μg
- : 1 mg/mL
Product Images
Western Blot Analysis:
Representative lot data.
Wild type HEK293 (lanes 1 and 2) cells or HEK293 cells transiently transfected with alpha J...
Western Blot Analysis:
Representative lot data.
Untreated human placenta lysate (lane 1) and lambda phosphatase treated human placenta lysat...
Anti-phospho-PLK1 (Ser137)
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R, B | WB | Rabbit | Affinity Purified | Polyclonal Antibody |
Description:
Anti-phospho-PLK1 (Ser137) Antibody | 07-1348
Promotional Text:
Specificity:
This antibody recognizes phosphorylated PLK1 at Ser137.
Molecular Weight:
68 kDa
Immunogen:
KLH-conjugated linear peptide corresponding to human phosphoylated PLK1 at and around Ser137.
Modifications:
Phosphorylation
Background Information:
Polo-like Kinase 1 (PLK1) is a serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Expressed PLK protein accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase.
Species Reactivity:
- Human
- Mouse
- Rat
- Bovine
Species Reactivity Note:
Human, Rat and Mouse. Predicted to react with Cow based on sequence homology.
Application Notes:
Western Blot (SNAP ID) Analysis: 0.5 µg/mL of this antibody detected PLK1 phosphorylated at Ser137 in human placenta tissue lysate.
Control:
Lambda phosphatase treated and untreated human placenta tissue lysate.
Quality Assurance:
Evaluated by Western Blot in human placenta tissue lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected PLK1 phosphorylated at Ser137 in lambda phosphatase treated and untreated human placenta tissue lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected PLK1 phosphorylated at Ser137 in lambda phosphatase treated and untreated human placenta tissue lysate.
Purification Method:
Affinity Purfied
Presentation:
Affinity purified rabbit serum in 0.1M Tris-Glycine (pH7.4), 150mM NaCl with 0.05% sodium azide.
Storage Conditions:
Maintain refrigerated at 2-8°C for up to 12 months.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- EC 2.7.11.21
- PLK
- PLK-1
- STPK13
Alternate Names:
- Polo-like kinase 1
- Serine/threonine-protein kinase 13
- cell cycle regulated protein kinase
- polo (Drosophia)-like kinase
- polo like kinase
- polo-like kinase (Drosophila)
- polo-like kinase 1 (Drosophila)
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Western Blotting
UniProt Summary:
FUNCTION: Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis.
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
ENZYME REGULATION: Activated by serine and threonine phosphorylation.
SUBUNIT STRUCTURE: Interacts with CEP170 and EVI5. Interacts and phosphorylates ERCC6L. Interacts with FAM29A. Interacts with BTBD12/SLX4. Ref.11 Ref.12 Ref.13 Ref.14 Ref.18
SUBCELLULAR LOCATION:Nucleus.
TISSUE SPECIFICITY:Placenta and colon.
DEVELOPMENTAL STAGE:Accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase.
INDUCTION: By growth-stimulating agents.
PTM: Catalytic activity is enhanced by phosphorylation of Thr-210 and/or Ser-137.Autophosphorylation and phosphorylation of Ser-137 are not significant events during activation of PLK1 in M phase.
SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
Contains 2 POLO box domains.
Contains 1 protein kinase domain.
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
ENZYME REGULATION: Activated by serine and threonine phosphorylation.
SUBUNIT STRUCTURE: Interacts with CEP170 and EVI5. Interacts and phosphorylates ERCC6L. Interacts with FAM29A. Interacts with BTBD12/SLX4. Ref.11 Ref.12 Ref.13 Ref.14 Ref.18
SUBCELLULAR LOCATION:Nucleus.
TISSUE SPECIFICITY:Placenta and colon.
DEVELOPMENTAL STAGE:Accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase.
INDUCTION: By growth-stimulating agents.
PTM: Catalytic activity is enhanced by phosphorylation of Thr-210 and/or Ser-137.Autophosphorylation and phosphorylation of Ser-137 are not significant events during activation of PLK1 in M phase.
SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
Contains 2 POLO box domains.
Contains 1 protein kinase domain.
Product Name:
Anti-phospho-PLK1 (Ser137)
Concentration:
1 mg/mL
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 μg
Format:
Affinity Purified
Host:
Rabbit