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Ordering Information
- : 07-1385
- : 100 μL
Product Images
Western Blot Analysis:
Tera-2 embryonic carcinoma cell lysate was resolved by SDS-PAGE, transferred to PVDF, and probed with Anti-Lin28, (1:1,000...
Anti-Lin28
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, IP | Rabbit | Serum | Polyclonal Antibody |
Description:
Anti-Lin28
Specificity:
Detects Lin28
Molecular Weight:
25 kDa
Immunogen:
Full-length His-tagged Lin28 recombinant protein (human).
Background Information:
Lin28 is a developmentally regulated RNA binding protein (RBP). Its down regulation is mediated in part by Lin4 and miR-125b, the mammalian micro-RNA homolog of Lin4. It is known to be negatively regulated by retinoic acid and act as a translational enhancer by driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis by increasing the number of initiation events per mRNA molecule. Lin28 is necessary and sufficient for selectively blocking the cleavage processing of pri-let-7 miRNAs in embryonic cells (Viswanathan, 2008). Lin28 is a negative regulator of miRNA biogenesis and may play a role in blocking miRNA-mediated differentiation in stem cells and in certain cancers (Viswanathan, 2008). The mechanism by which miR-125b represses Lin28 in mammalian cells appears to involve reductions not only in translational efficiency, but also in mRNA abundance (Wu, 2005). Lin28 has shown to be essential for skeletal muscle differentiation through the translational upregulation of IGF2 expression. It is known to be expressed in testis, placenta, and embryonic stem (ES) cells.
Species Reactivity:
- Human
- Mouse
Species Reactivity Note:
Human and Mouse. Other species have not been tested.
Application Notes:
Immunoprecipitation:
Independent lab validated this antibody in immunoprecipitation. The antibody immunoprecipitated Lin28 from Tera-2 embryonic carcinoma and mouse ES cells using 2μL of antibody per reaction.
Independent lab validated this antibody in immunoprecipitation. The antibody immunoprecipitated Lin28 from Tera-2 embryonic carcinoma and mouse ES cells using 2μL of antibody per reaction.
Quality Assurance:
Western Blot Analysis:
This lot detected Lin28 at 1:1,000 dilution in Tera-2 embryonic carcinoma cell lysate resolved via SDS-PAGE and transferred to PVDF.
This lot detected Lin28 at 1:1,000 dilution in Tera-2 embryonic carcinoma cell lysate resolved via SDS-PAGE and transferred to PVDF.
Purification Method:
Unpurified
Presentation:
Rabbit Serum
Storage Conditions:
Stable for 1 year at -20°C from date of receipt. Store at -80°C for longer term storage. Avoid repeated freeze/thaws.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- LIN-28
- LIN28A
Alternate Names:
RNA-binding protein LIN-28
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Western Blotting
- Immunoprecipitation
UniProt Summary:
FUNCTION: Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression By similarity.
SUBUNIT STRUCTURE: During skeletal muscle differentiation, associated with translation initiation complexes in the polysomal compartment. Directly interacts with EIF3S2. Interaction with NCL is RNA-dependent By similarity.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus › nucleolus. Note= Nucleolar localization observed in 10 - 15% of the nuclei in differentiated myotubes By similarity.
SPECIFICITY: Expressed in embryonic stem cells (ES cells), placenta and testis. Ref.7 Ref.8 Ref.9
DEVELOPMENTAL STAGE: Expressed in fetal liver. Expression decreases during differentiation of ES cells or upon induction of neuronal differentiation by retinoic acid. Ref.7 Ref.8 Ref.9
INDUCTION: Can be negatively regulated by the interaction of microRNAs miR-125a and miR-125b with at least two miRNA responsive elements (miREs) in the 3'-UTR of this gene. These interactions may reduce both translation efficiency and mRNA abundance. Negatively regulated by retinoic acid. Ref.6 Ref.10
DOMAIN: The CSD domain is required for function in muscle differentiation By similarity.
SIMILARITY: Belongs to the lin-28 family.
Contains 2 CCHC-type zinc fingers.
Contains 1 CSD (cold-shock) domain.
SUBUNIT STRUCTURE: During skeletal muscle differentiation, associated with translation initiation complexes in the polysomal compartment. Directly interacts with EIF3S2. Interaction with NCL is RNA-dependent By similarity.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus › nucleolus. Note= Nucleolar localization observed in 10 - 15% of the nuclei in differentiated myotubes By similarity.
SPECIFICITY: Expressed in embryonic stem cells (ES cells), placenta and testis. Ref.7 Ref.8 Ref.9
DEVELOPMENTAL STAGE: Expressed in fetal liver. Expression decreases during differentiation of ES cells or upon induction of neuronal differentiation by retinoic acid. Ref.7 Ref.8 Ref.9
INDUCTION: Can be negatively regulated by the interaction of microRNAs miR-125a and miR-125b with at least two miRNA responsive elements (miREs) in the 3'-UTR of this gene. These interactions may reduce both translation efficiency and mRNA abundance. Negatively regulated by retinoic acid. Ref.6 Ref.10
DOMAIN: The CSD domain is required for function in muscle differentiation By similarity.
SIMILARITY: Belongs to the lin-28 family.
Contains 2 CCHC-type zinc fingers.
Contains 1 CSD (cold-shock) domain.
Qty/Pk:
100 μL
Format:
Serum
Host:
Rabbit
Product Name:
Anti-Lin28
Antibody Type:
Polyclonal Antibody