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Ordering Information

  • : 07-158
  • : 200 µL
  • Product Family Information

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Immunohistochemistry Analysis:
Representative lot data.
Paraffin-embedded mouse embryo vascular endothelium tissue was prepared using heat...

Western Blotting Analysis:
Representative lot data.
PAE cells transfected with CSF/VEGFR2 chimera and treated with CSF was probed with Ant...

Anti-KDR/Flk-1/VEGFR2 Antibody

Species Reactivity Key Applications Host Format Antibody Type
M WB, IHC Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-KDR/Flk-1/VEGFR2 Antibody
Promotional Text:
Trade Name:
Upstate (Millipore)
Specificity:
This antibody recognizes KDR/Flk-1/VEGFR2 at the cytoplasmic domain.
Molecular Weight:
~150/180 kDa observed. 180 kDa is the mature form and 150 kDa is the immature unmodified form. In human and mouse cells the mature form of VEGFR2 is ~220-225 kDa. Faint band at ~225 kDa may be endogenous VEGFR2 in PAE cells.
Epitope:
Cytoplasmic domain
Immunogen:
GST-tagged recombinant protein corresponding to mouse KDR/Flk-1/VEGFR2 at the cytoplasmic domain.
Background Information:
The Flt gene family of receptor tyrosine kinases includes Flt-1, KDR/Flk-1, Flt-3, and Flt-4. They encode receptors for VEGF, characterized by seven Ig-loops within their extracellular domain and a split kinase domain within the cytoplasmic domain. VEGF is an important angiogenic factor of many solid tumors and is also involved in the differentiation of endothelial cells. Flt-1 (Fms-like tyrosine kinase, VEGF-R1) is expressed on endothelial cells and on the common precursors of endothelial and hematopoietic stem cells. KDR (Flk-1, VEGF-R2) is the principal receptor involved in VEGF signaling. KDR is expressed on endothelial cells and stimulates angiogenesis, but is also expressed on bone marrow cells, and is thought to play a role in driving the proliferation of most or all leukemic cells. Flt3 is a class III receptor tyrosine kinase that is closely related to c-Kit and c-Fms. It is expressed in primitive hematopoietic cells and its ligand exerts proliferative effects on these cells in co-operation with other cytokines. The VEGF receptors stimulate the Ras-MAP kinase pathway, suggesting that it signals as a conventional receptor tyrosine kinase.
Species Reactivity:
Mouse
Species Reactivity Note:
Demonstrated to react with Mouse. Expected to react with Rat based on a 99% sequence homology and Human based on 92% sequence homology.
Application Notes:
Immunohistochemistry Analysis: 1:100 dilution from a representative lot detected KDR/Flk-1/VEGFR2 in mouse embryo vascular endothelium tissue.
Control:
PAE cells transfected with CSF/VEGFR2 chimera and treated with CSF
Quality Assurance:
Evaluated by Western Blot in PAE cells transfected with CSF/VEGFR2 chimera and treated with CSF.

Western Blot Analysis: 1:1,000 dilution of this antibody detected KDR/Flk-1/VEGFR2 on 10 µg of PAE cells transfected with CSF/VEGFR2 chimera and treated with CSF.
Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
  • KDR
  • VEGFR-2
  • VEGFR2
  • FLK1
  • VEGFR
  • CD309
Alternate Names:
  • Vascular endothelial growth factor receptor 2
  • VEGFR-2
  • Fetal liver kinase 1
  • FLK-1
  • Kinase insert domain receptor
  • Protein-tyrosine kinase receptor flk-1
  • CD309
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
  • Western Blotting
  • Immunohistochemistry
UniProt Summary:
FUNCTION: Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

SIZE: 1356 amino acids; 151527 Da

SUBUNIT: Interacts with SHB upon VEGF activation. Interacts with HIV-1 Tat.

SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.

SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. & Contains 7 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 protein kinase domain.
Product Name:
Anti-KDR/Flk-1/VEGFR2 Antibody
Antibody Type:
Polyclonal Antibody
Qty/Pk:
200 µL
Format:
Affinity Purified
Host:
Rabbit
Applications:
This KDR/Flk-1/VEGFR2 antibody is validated for use in WB & IHC for the detection of the KDR/Flk-1/VEGFR2 protein.

Product Resources

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