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Immunofluorescent ICC Analysis:
HeLa cells were fixed, permeabilized, and stained using Anti-HuR (Cy3, Red). Cells were co-stained for actin (Pha...
Immunofluorescent ICC Analysis:
NIH/3T3, A431, and HeLa cells (Top, Middle, and Bottom, respectively) were grown, fixed, and permeabilized. The c...
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Anti-HuR
Recommended Replacement for: 07-1735 is recommended replacement for AB5831.| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, IF | Rabbit | Affinity Purified | Polyclonal Antibody |
Description:
Anti-HuR
Replaces:
07-1735 is recommended replacement for AB5831.
Specificity:
Detects HuR
Molecular Weight:
36 kDa
Epitope:
N-terminus
Immunogen:
Synthetic peptide corresponding to the N-terminus of human HuR.
Background Information:
HuR (Hu-antigen R, ELAV-like protein 1, ELAV1) is an RNA bindnig protein that contains 3 RNA-binding domains. It is known to binds AU-rich elements if mRNA. It regulates gene expression by destabilizing their mRNA. It is known to be upregulated in certain cancers such as breast and gastric cancers. Some of its targets include cFos, IL-3, and BRCA1 mRNAs. HuR has also been shown to be involved in the regulation of VEFG-A and NFkB expression in certain cell types. It activation and accumulation are also linked to many energy signaling molcules such as that of the PI3K/Akt pathway and AMPK.
Species Reactivity:
- Human
- Mouse
- Rat
Species Reactivity Note:
Human, Mouse, Rat. Other species have not been tested.
Application Notes:
Immunofluorescent ICC Analysis:
Control:
Jurkat cell lysate
Quality Assurance:
Western Blot Analysis:
The antibody detected HuR at 1:500-1:1,000 dilutions in Jurkat cell lysate resolved via SDS-PAGE and transferred to PVDF.
The antibody detected HuR at 1:500-1:1,000 dilutions in Jurkat cell lysate resolved via SDS-PAGE and transferred to PVDF.
Purification Method:
Affinity purified polyclonal precipitated in ammonium sulfate.
Presentation:
Rabbit polyclonal precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- ELAV1
- HUR
- HuR
- Hua
- MelG
Alternate Names:
- ELAV-like 1
- Hu antigen R
- Hu-antigen R
- embryonic lethal, abnormal vision, drosophila, homolog-like 1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
The protein encoded by this gene is a member of the ELAVL protein family. This encoded protein contains 3 RNA-binding domains and binds cis-acting AU-rich elements. It destabilizes mRNAs and thereby regulates gene expression. [provided by RefSeq]
Key Applications:
- Western Blotting
- Immunofluorescence
UniProt Summary:
FUNCTION: SwissProt: Q15717 # Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs.
SIZE: 326 amino acids; 36092 Da
SUBUNIT: Interacts with ANP32A.
TISSUE SPECIFICITY: Ubiquitous.
PTM: Methylated at Arg-217 by CARM1 in macrophages in response to LPS challenge.
SIMILARITY: SwissProt: Q15717 ## Belongs to the RRM elav family. & Contains 3 RRM (RNA recognition motif) domains.
SIZE: 326 amino acids; 36092 Da
SUBUNIT: Interacts with ANP32A.
TISSUE SPECIFICITY: Ubiquitous.
PTM: Methylated at Arg-217 by CARM1 in macrophages in response to LPS challenge.
SIMILARITY: SwissProt: Q15717 ## Belongs to the RRM elav family. & Contains 3 RRM (RNA recognition motif) domains.
Qty/Pk:
100 μL
Format:
Affinity Purified
Host:
Rabbit
Product Name:
Anti-HuR
Antibody Type:
Polyclonal Antibody