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Ordering Information

  • : 07-175
  • : 200 μg
  • : Please refer to the Certificate of Analysis for the lot-specific concentration.

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Immunoblot Analysis: 10 μg of untreated (lane 1) or anisomycin treated (30 min) (lane 2) NIH/3T3 cell lysates were resolved by electrophoresis,...

Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223 )

Species Reactivity Key Applications Host Format Antibody Type
M, H, R, Ca, Xn, Ch, B, Eq WB, EA, IP Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) Antibody
Promotional Text:
Specificity:
This antibody recognizes JNK when phosphorylated at Thr183/Tyr185 and Thr221/Tyr223 .
Molecular Weight:
~ 42 kDa observed. There are several isoforms between 40-50 kDa.
Epitope:
Phosphorylated at Thr183/Tyr185 and Thr221/Tyr223
Immunogen:
KLH-conjugated linear peptide corresponding to human JNK phosphorylated at Thr183/Tyr185 and Thr221/Tyr223.
Modifications:
Phosphorylation
Isotype:
Background Information:
JNK1(MAPK8) is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-α) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrome c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. JNK1 is activated by threonine and tyrosine phosphorylation by either of two dual specificity kinases, MAP2K4 and MAP2K7. The JNK pathway is critically involved in diabetes and levels are abnormally elevated in obesity. The cell-permeable JNK inhibitory peptide may have promise as a therapeutic agent for diabetes.
Species Reactivity:
  • Mouse
  • Human
  • Rat
  • Canine
  • Xenopus
  • Chicken
  • Bovine
  • Horse
Species Reactivity Note:
Demonstrated to react with mouse and human. Predicted to react with rat, canine, xenopus, chicken, bovine, and equine based on 100% sequence homology.
Application Notes:
Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated JNK phosphorylated at Thr183/Tyr185 and Thr221/Tyr223 from RAW264.7 cell lysate.
Control:
Untreated and Lambda phosphatase treated RAW264.7 cell lysates
Quality Assurance:
Evaluated by Western Blot in untreated and lambda phosphatase treated RAW264.7 cell lysates.

Western Blot Analysis: 2 µg/mL of this antibody detected JNK on 10 µg of untreated and lambda phosphatase RAW264.7 cell lysates.
Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
  • MAPK8
  • JNK1
  • JNK21B1/2
  • PRKM8
  • JNK-46
  • JNK
  • SAPK1
  • JNK1A2
Alternate Names:
  • JNK1 alpha protein kinase
  • JNK1 beta protein kinase
  • JUN N-terminal kinase
  • Stress-activated protein kinase JNK1
  • Stress-activated protein kinase JNK1
  • mitogen-activated protein kinase 8
  • mitogen-activated protein kinase 8 isoform JNK1 alpha1
  • mitogen-activated protein kinase 8 isoform JNK1 alpha1
  • protein kinase JNK1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
  • Western Blotting
  • Enzyme Assay
  • Immunoprecipitation
Entrez Gene Summary:
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.
UniProt Summary:
FUNCTION: JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta- 2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.

COFACTOR: Magnesium.

SIZE: 427 amino acids; 48296 Da

SUBUNIT: Binds to at least four scaffolding proteins, MAPK8IP1/JIP-1, MAPK8IP2/JIP-2, MAPK8IP3/JIP-3/JSAP1 and SPAG9/MAPK8IP4/JIP-4. These proteins also bind other components of the JNK signaling pathway. Interacts with TP53 and WWOX. Interacts with JAMP (By similarity).

DOMAIN: The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

PTM: Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.

SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain. ... hide » see more »
Product Name:
Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223 )
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Polyclonal Antibody
Qty/Pk:
200 μg
Format:
Affinity Purified
Host:
Rabbit
Applications:
Detect phospho-JNK (Thr183/Tyr185 using this Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) Antibody validated for use in WB, EA & IP.

Product Resources

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