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WB: Rat liver cell lysate was probed with anti-AMP Activated Protein Kinase α-pan
Anti-AMPK α-pan
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R | WB | Rabbit | Affinity Purified | Polyclonal Antibody |
Description:
Anti-AMPK α-pan
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes AMP-activated protein kinase alpha subunits.
Molecular Weight:
63 kDa
Immunogen:
human AMP-activated protein a1 catalytic subunit (AEKQKHDGRVKIGHYILGD)
Isotype:
IgG
Species Reactivity:
- Human
- Rat
Control:
Rat liver preparation
Quality Assurance:
routinely evaluated by immunoblot on a rat liver protein preparation
Purification Method:
Immunoaffinity purified
Presentation:
Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
Storage Conditions:
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- PRKAA1
- MGC57364
- AMPK
- AMPK1
- MGC33776
- AMPKa1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Key Applications:
Western Blotting
UniProt Summary:
FUNCTION: SwissProt: Q13131 # Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
COFACTOR: Magnesium.
SIZE: 550 amino acids; 62808 Da
SUBUNIT: Heterotrimer of an alpha catalytic subunit, a beta and a gamma non-catalytic subunits. Interacts with FNIP1.
SIMILARITY: SwissProt: Q13131 ## Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. & Contains 1 protein kinase domain.
COFACTOR: Magnesium.
SIZE: 550 amino acids; 62808 Da
SUBUNIT: Heterotrimer of an alpha catalytic subunit, a beta and a gamma non-catalytic subunits. Interacts with FNIP1.
SIMILARITY: SwissProt: Q13131 ## Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. & Contains 1 protein kinase domain.
Qty/Pk:
200 μg
Brand Family:
Upstate
Format:
Affinity Purified
Host:
Rabbit
Product Name:
Anti-AMPK α-pan
Antibody Type:
Polyclonal Antibody