Anti-phospho-PLCγ-1 Antibody (Tyr783)

Anti-phospho-PLCγ-1 (Tyr783)

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This antibody recognizes phospho-PLCγ-1 (Tyr783).

135 kDa

KLH conjugated linear peptide corresponding to phospho-PLCγ-1 (Tyr783).

Phosphorylation

Phospholipase C gamma 1 is a tyrosine kinase substrate for many receptor and nonreceptor tyrosine kinases. Activation of the enzyme produces two second messenger molecules, inositol 1,4,5-trisphosphate and diacylglycerol, which provoke the mobilization of intracellular Ca2+ and activation of protein kinase C. Although the mammalian genome encodes 10 known phosphoinositide specific PLCs, only the gamma 1 and gamma 2 isoforms are regulated by tyrosine kinase activity. PLC beta isoform activity is controlled by heterotrimeric G protein coupled receptors, while the mechanism of regulation of PLCgamma activity is unknown.

• Human
• Mouse
• Canine
• Horse
• Rat

Proven to react with human. Predicted to react with Mouse, Rat, Equine, and Canine based on sequence homology.

Western Blot SNAP ID: 1:1,000 dilution of this antibody from a previous lot detected phospho-PLCγ-1 (Tyr783) on of untreated and EGF treated A431 cell lysate.
Western Blot SNAP ID Analysis:

A431 cell lysate

Evaluated by Western Blot in A431 cell lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected phospho-PLCγ-1 (Tyr783) on untreated and EGF treated A431 cell lysate.

Affinity Purfied

Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-glycine, pH 7.4, 150 mM NaCl and 0.05% sodium azide.

Maintain refrigerated at 2-8°C for up to 6 months.

P19174

NP_002651

• 1-phosphatidyl-D-myo-inositol-4,5-bisphosphate
• EC 3.1.4.11
• OTTHUMP00000031787
• OTTHUMP00000178982
• PLC-148
• PLC-II
• PLC-gamma-1
• PLC1
• PLC148
• PLCgamma1
• inositoltrisphosphohydrolase

• 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1
• Phosphoinositide phospholipase C
• Phospholipase C-gamma-1
• monophosphatidylinositol phosphodiesterase
• phosphatidylinositol phospholipase C
• phosphoinositidase C
• phospholipase C gamma 1
• phospholipase C, gamma 1
• phospholipase C, gamma 1 (formerly subtype 148)
• phospholipase C-148
• triphosphoinositide phosphodiesterase

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene.

Western Blotting

FUNCTION: PLC-gamma is a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.

CATALYTIC ACTIVITY: 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

COFACTOR: Calcium.

SUBUNIT STRUCTURE: Interacts with AGAP2 via its SH3 domain By similarity. Interacts with phosphorylated LAT upon TCR activation. Interacts with the Pro-rich domain of TNK1 via its SH3 domain. Associates with BLNK, VAV1, GRB2 and NCK1 in a B-cell antigen receptor-dependent fashion. Interacts with CBLB in activated T-cells; which inhibits phosphorylation. Interacts with SHB. Interacts via its SH3 domain with the Arg/Gly-rich-flanked Pro-rich domains of KHDRBS1/SAM68. This interaction is selectively regulated by arginine methylation of KHDRBS1/SAM68. Interacts with INPP5D/SHIP1 and CLNK By similarity. Interacts with RALGPS1. Interacts (via SH3 domain) with HEV ORF3 protein.

DOMAIN: The SH3 domain mediates interaction with CLNK By similarity. The SH3 domain also mediates interaction with RALGPS1.

PTM: The receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors.

Ubiquitinated by CBLB in activated T-cells By similarity.

SEQUENCE SIMILARITIES: Contains 1 C2 domain.

Contains 1 EF-hand domain.

Contains 2 PH domains.

Contains 1 PI-PLC X-box domain.

Contains 1 PI-PLC Y-box domain.

Contains 2 SH2 domains.

Contains 1 SH3 domain.

Anti-phospho-PLCγ-1 (Tyr783)

0.5 mg/mL

Polyclonal Antibody

100 μL

Affinity Purified

Rabbit

This phospho-PLCγ-1 antibody is validated for use in WB for the detection of the phospho-PLCγ-1 protein.