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Ordering Information
- : 07-219
- : 100 μg
- : 0.5 mg/mL
Product Images
Immunocytochemistry Analysis:
Representative lot data.
Confocal fluorescent analysis of NIH/3T3, HeLa, A431 cells using Anti-Histone Macro...
Western Blotting Analysis:
Representative lot data.
HeLa acid extract was probed with Anti-Histone Macro H2A.1 (0.2 µg/mL).
Protein...
Anti-Histone Macro H2A.1
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, IC | Rabbit | Affinity Purified | Polyclonal Antibody |
Description:
Anti-Histone Macro H2A.1
Qty/Pk:
100 μg
Background Information:
Histone MacroH2A (mH2A) is a variant histone also known as H2AFY. mH2A is enriched in chromatin of the female inactive X chromosome and in highly methylated CpG loci. This histone variant is known to repress RNA polymerase II-driven transcription at the level of transcriptional initiation. In contrast to most histone variants containing only subtle changes, mH2A contains a large non-histone domain (the macro domain) on its C-terminus, resulting in a histone approximately three times the size of conventional H2A. There are 3 isoforms of mH2A, two of which (mH2A1.1, mH2A1.2 ) are formed by alternative splicing and another (mH2A2) from a separate gene. There are two phosphorylation sites within the basic hinge region of mH2A: Thr128 and Ser137. Phospho-Ser137 is enriched in mitosis, implicating mH2A in the regulation of posttranslational modifications throughout the cell cycle.
Key Applications:
- Western Blotting
- Immunocytochemistry
Application Notes:
Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Histone MacroH2A.1 in NIH/3T3, A431, and HeLa cells.
Species Reactivity:
- Human
- Mouse
Species Reactivity Note:
Demonstrated to react with human and mouse.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
UniProt Summary:
FUNCTION: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces an hypoacetylated state of chromatin. In addition, isoform 1, but not isoform 2, binds ADP-ribose and O-acetyl-ADP-ribose, and may be involved in ADP-ribose-mediated chromatin modulation.
SUBUNIT STRUCTURE: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. Interacts with SPOP. Part of a complex consisting of H2AFY, CUL3 and SPOP. Interacts with HDAC1 and HDAC2 (By similarity).
SUBCELLULAR LOCATION: Nucleus. Chromosome. Note: Enriched in inactive X chromosome chromatin and in senescence-associated heterochromatin. In quiescent lymphocytes, associated with centromeric constitutive heterochromatin.
TISSUE SPECIFICTY: Ubiquitously expressed, with high levels in testis. Present in liver, kidney and adrenal gland (at protein level). In the liver, present in hepatocytes and at a lesser extent in cells of the bile ducts. In the kidney, present in proximal and distal convoluted tubules and in straight proximal tubules. In the adrenal gland, present in inner cells of the cortex and medulla.
PTM: Monoubiquitinated at either Lys-116 or Lys-117. May also be polyubiquitinated. Ubiquitination is mediated by the CUL3/SPOP E3 complex and does not promote proteasomal degradation. Instead, it is required for enrichment in inactive X chromosome chromatin (By similarity).
SEQUENCE SIMILARITIES: Contains 1 histone H2A domain.
Contains 1 Macro domain.
SUBUNIT STRUCTURE: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. Interacts with SPOP. Part of a complex consisting of H2AFY, CUL3 and SPOP. Interacts with HDAC1 and HDAC2 (By similarity).
SUBCELLULAR LOCATION: Nucleus. Chromosome. Note: Enriched in inactive X chromosome chromatin and in senescence-associated heterochromatin. In quiescent lymphocytes, associated with centromeric constitutive heterochromatin.
TISSUE SPECIFICTY: Ubiquitously expressed, with high levels in testis. Present in liver, kidney and adrenal gland (at protein level). In the liver, present in hepatocytes and at a lesser extent in cells of the bile ducts. In the kidney, present in proximal and distal convoluted tubules and in straight proximal tubules. In the adrenal gland, present in inner cells of the cortex and medulla.
PTM: Monoubiquitinated at either Lys-116 or Lys-117. May also be polyubiquitinated. Ubiquitination is mediated by the CUL3/SPOP E3 complex and does not promote proteasomal degradation. Instead, it is required for enrichment in inactive X chromosome chromatin (By similarity).
SEQUENCE SIMILARITIES: Contains 1 histone H2A domain.
Contains 1 Macro domain.
Molecular Weight:
~ 40 kDa observed
Quality Assurance:
Evaluated by Western Blot in HeLa acid extract.
Western Blot Analysis: 0.2 µg/mL of this antibody detected histone MacroH2A.1 on 10 µg of HeLa acid extract.
Western Blot Analysis: 0.2 µg/mL of this antibody detected histone MacroH2A.1 on 10 µg of HeLa acid extract.
Immunogen:
GST-tagged recombinant protein corresponding to mcro domain of mouse Histone MacroH2A.1.
Control:
HeLa acid extract
Format:
Affinity Purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
UniProt Number:
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
Host:
Rabbit
Epitope:
Macro domain
Gene Symbol:
- H2A.y
- H2AF12M
- H2AFJ
- H2A/y
- MACROH2A1.1
- mH2A
- MACROH2A
Product Name:
Anti-Histone Macro H2A.1
Entrez Gene Number:
Alternate Names:
- H2A histone family, member Y
- histone macroH2A1.2
- macroH2A1.2
- Histone H2A.y
- Histone macroH2A
- histone macroH2A1.1
- Medulloblastoma antigen MU-MB-50.205
- core histone macro-H2A.1
Concentration:
0.5 mg/mL
Antibody Type:
Polyclonal Antibody
Purification Method:
Affinity purified
Specificity:
This antibody recognizes Histone MacroH2A.1 at the macro domain.