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WB: Mouse brain membrane protein preparation probed with anti-TrkB (1 microgram/ml).
Anti-TrkB
| Reatividade à Espécie | Aplicações Essenciais | Hospedeiro | Formato | Tipo de Anticorpo |
|---|---|---|---|---|
| R, M | WB | Rabbit | Purified | Polyclonal Antibody |
Número Uniprot:
UniProt Summary
FUNCTION: SwissProt: Q16620 # Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC- gamma-1.
SIZE: 822 amino acids; 91999 Da
SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (h ... see more »
SIZE: 822 amino acids; 91999 Da
SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (h ... see more »
Número Genbank:
Entrez Gene Summary
Receptor for brain-derived neurotrophic factor (BDNF) [RGD].
Description:
Anti-TrkB
Background Information:
The Trk (tropomyosin-receptor-kinase) proto-oncogene family of receptor tyrosine kinases contains four members, TrkA, TrkB, TrkC, and TrkE, which are variably expressed throughout the central and peripheral nervous systems. TrkB is the high-affinity to the binding brain-derived neurotrophic factor (BDNF) receptor and neurotrophin-4/5 NT-4/5). TrkB is autophosphorylated on five cytoplasmic tyrosines. The binding of BDNF to TrkB receptor causes many intercellular cascades to be activated such as promoting survival of sensory neurons mainly through Shc site-independent pathways.The signaling activity of these receptors includes the Ras – MAP kinase pathway, and the PI3 Kinase pathway. These receptors are responsible for resistance to apoptosis, obesity, Alzheimer’s disease, and mood disorders.
Alternate Names:
- BDNF/NT-3 growth factors receptor
- Neurotrophic tyrosine kinase receptor type 2
- TrkB tyrosine kinase
- neurotrophic tyrosine kinase, receptor, type 2
- tyrosine kinase receptor B
Specificity:
Recognizes TrkB, Mr 145 kDa, as well as an additional protein, Mr 90 kDa, which is likely a TrkB degradation product.
Key Applications:
Western Blotting
Species Reactivity:
- Rat
- Mouse
Species Reactivity Note:
Rat and mouse.
Isotype:
IgG
Immunogen:
The entire extracellular domain (corresponding to residues 1–429) of the rat TrkB receptor, expressed in COS cells. The immunizing sequence has 97% identity with mouse TrkB and 88% identity with human TrkB.
Molecular Weight:
145 κDa
Control:
Brain tissue.
Presentation:
Purified rabbit polyclonal in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide, and 30% glycerol.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Gene Symbol:
- NTRK2
- GP145-TrkB
- TRKB
- Trk-B
Quality Assurance:
Evaluated by western blot on mouse brain membrane protein preparations.
Western Blot Analysis:
0.5-2 µg/mL of this antibody detected TrkB in mouse brain membrane protein preparations.
Western Blot Analysis:
0.5-2 µg/mL of this antibody detected TrkB in mouse brain membrane protein preparations.
Antibody Category:
Neuroscience
Antibody Sub-Category:
Neurochemistry & Neurotrophins
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Upstate (Millipore)
Format Code:
Pur
Concentration:
1 mg/mL
Antibody Type:
Polyclonal Antibody
Format:
Purified
Host:
Rabbit
Purification Method:
Protein A Purified