Ordering Information
- : 07-276
- : 200 μL
- : Variable
Product Images
WB: 3T3/NIH cell lysate probed with Anti-PDGF Receptor α (1:500 dilution).
Anti-PDGFRα Antibody | 07-276
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | IP, WB | Rabbit | Affinity Purified | Polyclonal Antibody |
Description:
Anti-PDGFRα Antibody | 07-276
Promotional Text:
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes PDGF Receptor alpha.
Molecular Weight:
150/170 kDa (doublet)
Immunogen:
peptide corresponding to amino acids 1035-1053 (C-GKRNRHSSQTSEESAIETG) of human PDGF Receptor α
Isotype:
IgG
Background Information:
Platelet derived growth factor receptor (PDGF Receptor) is a transmembrane glycoprotein of 170-185 kDa which undergoes homo or heterodimerization into complexes of alpha and beta subunits upon ligand binding, depending on the isoform of PDGF (PDGF AA, BB or AB) that binds. The phosphorylation of tyrosine residues in the now activated receptor can control multiple signaling events such as actin reorganization, transcription, cell growth, migration and differentiation, and also lead to activation of the Ras, Raf and ERK 1 and 2 pathway.
Species Reactivity:
- Human
- Mouse
Control:
HeLa cells, extracts from 3T3/NIH or HepG2 cells, human brain tissue
Quality Assurance:
routinely evaluated by immunoblot on 3T3/NIH cell lysate
Purification Method:
Immunoaffinity purified
Presentation:
Protein A Purified immunoglobulin in 0.14 M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- PDGFRA
- CD140a
- PDGF-R-alpha
- MGC74795
- PDGFR2
- Rhe-PDGFRA
- CD140A
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Immunoprecipitation
- Western Blotting
Entrez Gene Summary:
This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.
UniProt Summary:
FUNCTION: SwissProt: P16234 # Receptor that binds both PDGFA and PDGFB and has a tyrosine-protein kinase activity.
SIZE: 1089 amino acids; 122670 Da
SUBUNIT: Homodimer, and heterodimer with PDGFRB. Interacts with SHB (By similarity).
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
DISEASE: SwissProt: P16234 # A fusion of PDGFRA and FIP1L1 (FIP1L1-PDGFRA), due to an interstitial chromosomal deletion, is the cause of some cases of hypereosinophilic syndrome (HES) [MIM:607685]. HES is a rare hematologic disorder characterized by sustained overproduction of eosinophils in the bone marrow, eosinophilia, tissue infiltration and organ damage.
SIMILARITY: SwissProt: P16234 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. & Contains 5 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 protein kinase domain.
SIZE: 1089 amino acids; 122670 Da
SUBUNIT: Homodimer, and heterodimer with PDGFRB. Interacts with SHB (By similarity).
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
DISEASE: SwissProt: P16234 # A fusion of PDGFRA and FIP1L1 (FIP1L1-PDGFRA), due to an interstitial chromosomal deletion, is the cause of some cases of hypereosinophilic syndrome (HES) [MIM:607685]. HES is a rare hematologic disorder characterized by sustained overproduction of eosinophils in the bone marrow, eosinophilia, tissue infiltration and organ damage.
SIMILARITY: SwissProt: P16234 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. & Contains 5 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 protein kinase domain.
Brand Family:
Upstate
Product Name:
Anti-PDGFRα Antibody | 07-276
Concentration:
Variable
Antibody Type:
Polyclonal Antibody
Qty/Pk:
200 μL
Format:
Affinity Purified
Host:
Rabbit