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WB: 20 micrograms of mouse heart cytosol cell lysate was probed with Anti-phospho-AcCoA Carboxylase.
Anti-phospho-Acetyl CoA Carboxylase (Ser79)
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R, Ca, Ch, B | WB | Rabbit | null | Polyclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: Q13085 # Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.
COFACTOR: Biotin. & Binds 2 manganese ions per subunit.
SIZE: 2346 amino acids; 265554 Da
SUBUNIT: Interacts in its inactive phosphorylated form with the BRCT domains of BRCA1 which prevents ACACA dephosphorylation and inhibits lipid synthes ... see more »
COFACTOR: Biotin. & Binds 2 manganese ions per subunit.
SIZE: 2346 amino acids; 265554 Da
SUBUNIT: Interacts in its inactive phosphorylated form with the BRCT domains of BRCA1 which prevents ACACA dephosphorylation and inhibits lipid synthes ... see more »
Entrez Gene Number:
Entrez Gene Summary
Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylatio
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Description:
Anti-phospho-Acetyl CoA Carboxylase (Ser79)
Background Information:
Acetyl CoA carboxylase (ACC) is a biotin-dependent enzyme that catalyzes carboxylation of acetyl-CoA to produce malonyl-CoA through its two catalytic activities, biotin carboxylase (BC) and carboxyltransferase (CT). ACC is a multi-subunit enzyme in most prokaryotes, whereas it is a large, multi-domain enzyme in most eukaryotes. The activity of ACC can be controlled at the transcriptional level as well as by small molecule modulators and covalent modification. Human genome contains the genes for two different ACCs - ACACA and ACACB. The activity of the enzyme is controlled by the reversible phosphorylation. The activities of the enzyme is inhibited if phosphorylated; the phosphorylation takes place when the hormones, glucagon or epinephrine bind to the receptors or the energy status of the cell is low, leading to the activation of the AMP-activated protein kinase. The presence of fatty acid inhibits the activities of the enzyme. When insulin binds to its receptors of the cell, it activates a phosphatase to dephosphorylate the enzyme; the activities of the acetyl CoA carboxylase is thus enhanced. Acetyl CoA carboxylase has recently become a target in the design of new anti-obesity and antibiotic drugs.
Alternate Names:
- acetyl-CoA carboxylase 1
- acetyl-CoA carboxylase-alpha
- acetyl-Coenzyme A carboxylase alpha
Specificity:
Recognizes Acetyl CoA Carboxylase phosphorylated at Serine 79, MW 257 kDa.
Key Applications:
Western Blotting
Species Reactivity:
- Human
- Mouse
- Rat
- Canine
- Chicken
- Bovine
Species Reactivity Note:
Human. Expected to react with Cow, Dog, Chicken, Rat and Mouse based on immunogen sequence homology.
Immunogen:
KLH conjugated synthetic peptide (C-HM RSSM[pS]GLHLVK) corresponding to amino acid 73-85 of rat Acetyl CoA Carboxylase.
Epitope:
N-terminal
Modifications:
Phosphorylation
Molecular Weight:
257 kDa
Presentation:
Protein A purified in buffer containing in 0.1M Tris-Glycine (pH7.4), 150 mM NaCl, with 0.05% NaN3.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
Gene Symbol:
- ACACA
- ACC-alpha
- ACCA
- ACC
- ACC1
- ACAC
Quality Assurance:
Evaluated by Western Blot on Lambda phosphatase-treated and untreated A375 cell lysate.
Western Blot Analysis: 1:500 dilution of this antibody detected endogenous phospho-Acetyl CoA Carboxylase (Ser79) in lysate from Lambda phosphatase-treated and untreated A375 cells.
Western Blot Analysis: 1:500 dilution of this antibody detected endogenous phospho-Acetyl CoA Carboxylase (Ser79) in lysate from Lambda phosphatase-treated and untreated A375 cells.
Antibody Category:
Signaling
Antibody Sub-Category:
Insulin/Energy Signaling
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Upstate (Millipore)
Format Code:
Pur
Concentration:
0.5 mg/mL
Antibody Type:
Polyclonal Antibody
Host:
Rabbit
Purification Method:
Protein A chromatography