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WB: NIH-3T3 cell lysate, non-treated (lane 1) and PDGF-treated (lane 2) was probed with Anti-phospho-Akt1/PKBα (Ser473).
Anti-phospho-Akt1/PKBα (Ser473)
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H, Ch, R, Xn, B | WB | Rabbit | Purified | Polyclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: P31749 # General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Medi
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Entrez Gene Number:
Entrez Gene Summary
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuro
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Description:
Anti-phospho-Akt1/PKBα (Ser473)
Background Information:
The serine threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet derived growth factor. The activation is rapid and specific. In the developing nervous system AKT is a critical mediator of growth factor induced neuronal survival. Survival factors can suppress apoptosis in a transcription independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene (referenced from entrez gene).
Alternate Names:
- Protein kinase B
- RAC-alpha serine/threonine-protein kinase
- murine thymoma viral (v-akt) oncogene homolog-1
- rac protein kinase alpha
- v-akt murine thymoma viral oncogene homolog 1
Specificity:
Recognizes phosphorylated Akt1/PKBα, Mr 60 kDa.
Key Applications:
Western Blotting
Species Reactivity:
- Mouse
- Human
- Chicken
- Rat
- Xenopus
- Bovine
Species Reactivity Note:
Human and mouse. Predicted cross-reactivity with rat, bovine, chicken, and Xenopus based on immunogen sequence homology.
Isotype:
IgG
Immunogen:
KLH-conjugated, synthetic peptide containing a pSer that corresponds to amino acid position 473 of human Akt1. The immunizing sequence is identical in mouse and bovine Akt1. Akt2 and Akt3 share significant homology with the peptide immunogen sequence.
Epitope:
Ser473
Modifications:
Phosphorylation
Molecular Weight:
60 kDa
Control:
PDGF-treated 3T3/NIH cells.
Presentation:
Purified rabbit polyclonal IgG in buffer containing 0.07 M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide and 30% glycerol. Store at -20°C.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Gene Symbol:
- AKT1
- RAC
- PRKBA
- RAC-ALPHA
- PKB
- MGC99656
- RAC-PK-alpha
- C-AKT
- AKT
Quality Assurance:
Routinely evaluated by western blot on lysates from mouse NIH-3T3 fibroblasts treated with PDGF.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected phosphorylated Akt1/PKBα in lysates from mouse 3T3/NIH fibroblasts treated with 100 ng/mL PDGF for 20 minutes.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected phosphorylated Akt1/PKBα in lysates from mouse 3T3/NIH fibroblasts treated with 100 ng/mL PDGF for 20 minutes.
Antibody Category:
Signaling
Antibody Sub-Category:
PI3K, Akt, & mTOR Signaling
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Upstate (Millipore)
Format Code:
Pur
Concentration:
1 mg/mL
Antibody Type:
Polyclonal Antibody
Format:
Purified
Host:
Rabbit
Purification Method:
Protein A Purified